Miyawaki Jin, Matsumura Sachiko, Yuge Ryota, Murakami Tatsuya, Sato Shigeo, Tomida Akihiro, Tsuruo Takashi, Ichihashi Toshinari, Fujinami Takako, Irie Hiroshi, Tsuchida Kunihiro, Iijima Sumio, Shiba Kiyotaka, Yudasaka Masako
Japan Science and Technology Agency,Sanbancho Chiyoda-ku, Tokyo, Japan.
ACS Nano. 2009 Jun 23;3(6):1399-406. doi: 10.1021/nn9004846.
Single-walled carbon nanohorns (SWNHs) are single-graphene tubules that have shown high potential for drug delivery systems. In drug delivery, it is essential to quantitatively determine biodistribution and ultrastructural localization. However, to date, these determinations have not been successfully achieved. In this report, we describe for the first time a method that can achieve these determinations. We embedded Gd(2)O(3) nanoparticles within SWNH aggregates (Gd(2)O(3)@SWNHag) to facilitate detection and quantification. Gd(2)O(3)@SWNHag was intravenously injected into mice, and the quantities of Gd in the internal organs were measured by inductively coupled plasma atomic emission spectroscopy: 70-80% of the total injected material accumulated in liver. The high electron scattering ability of Gd allows detection with energy dispersive X-ray spectroscopy and facilitates the ultrastructural localization of individual Gd(2)O(3)@SWNHag with transmission electron microscopy. In the liver, we found that the Gd(2)O(3)@SWNHag was localized in Kupffer cells but were not observed in hepatocytes. In the Kupffer cells, most of the Gd(2)O(3)@SWNHag was detected inside phagosomes, but some were in another cytoplasmic compartment that was most likely the phagolysosome.
单壁碳纳米角（SWNHs）是单石墨烯微管，在药物递送系统中显示出巨大潜力。在药物递送中，定量确定生物分布和超微结构定位至关重要。然而，迄今为止，这些测定尚未成功实现。在本报告中，我们首次描述了一种能够实现这些测定的方法。我们将Gd₂O₃纳米颗粒嵌入SWNH聚集体（Gd₂O₃@SWNHag）中以促进检测和定量。将Gd₂O₃@SWNHag静脉注射到小鼠体内，通过电感耦合等离子体原子发射光谱法测量内脏器官中的Gd含量：注射的总物质中有70 - 80%积聚在肝脏中。Gd的高电子散射能力使得能够用能量色散X射线光谱法进行检测，并有助于通过透射电子显微镜对单个Gd₂O₃@SWNHag进行超微结构定位。在肝脏中，我们发现Gd₂O₃@SWNHag定位于库普弗细胞中，但在肝细胞中未观察到。在库普弗细胞中，大部分Gd₂O₃@SWNHag在吞噬体内被检测到，但有些在另一个细胞质区室中，很可能是吞噬溶酶体。
