Sano Mahoko, Izumiya Makoto, Haniu Hisao, Ueda Katsuya, Konishi Kosuke, Ishida Haruka, Kuroda Chika, Uemura Takeshi, Aoki Kaoru, Matsuda Yoshikazu, Saito Naoto
Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano 390-8621, Japan.
Biomedical Engineering Division, Graduate School of Science and Technology, Shinshu University, Nagano 390-8621, Japan.
Nanomaterials (Basel). 2020 Jul 14;10(7):1374. doi: 10.3390/nano10071374.
One of the greatest challenges to overcome in the pursuit of the medical application of carbon nanomaterials (CNMs) is safety. Particularly, when considering the use of CNMs in drug delivery systems (DDSs), evaluation of safety at the accumulation site is an essential step. In this study, we evaluated the toxicity of carbon nanohorns (CNHs), which are potential DDSs, using human lymph node endothelial cells that have been reported to accumulate CNMs, as a comparison to fibrous, multi-walled carbon nanotubes (MWCNTs) and particulate carbon black (CB). The effect of different surface characteristics was also evaluated using two types of CNHs (untreated and oxidized). In the fibrous MWCNT, cell growth suppression, as well as expression of inflammatory cytokine genes was observed, as in previous reports. In contrast, no significant toxicity was observed for particulate CB and CNHs, which was different from the report of CB cytotoxicity in vascular endothelial cells. These results show that (1) lymph endothelial cells need to be tested separately from other endothelial cells for safety evaluation of nanomaterials, and (2) the potential of CNHs as DDSs.
在追求碳纳米材料（CNMs）医学应用的过程中，需要克服的最大挑战之一是安全性。特别是在考虑将CNMs用于药物递送系统（DDSs）时，评估其在蓄积部位的安全性是至关重要的一步。在本研究中，我们使用据报道会蓄积CNMs的人淋巴结内皮细胞，评估了作为潜在DDSs的碳纳米角（CNHs）的毒性，并将其与纤维状多壁碳纳米管（MWCNTs）和颗粒状炭黑（CB）进行比较。还使用两种类型的CNHs（未处理的和氧化的）评估了不同表面特性的影响。在纤维状MWCNT中，如先前报道的那样，观察到细胞生长抑制以及炎性细胞因子基因的表达。相比之下，颗粒状CB和CNHs未观察到明显毒性，这与关于CB在血管内皮细胞中的细胞毒性的报道不同。这些结果表明：（1）在纳米材料安全性评估中，淋巴内皮细胞需要与其他内皮细胞分开进行测试；（2）CNHs作为DDSs的潜力。
