Helicos BioSciences Corporation, One Kendall Square Bldg 700, Cambridge, MA 02139, USA.
Genome Med. 2009 May 11;1(5):50. doi: 10.1186/gm50.
A large amount of experimental data collected over the last decade has shown that genomic organization is very complex and has highlighted the fact that the current set of gene annotations does not fully capture this complexity. Much of the RNA detected in a cell is found to originate from outside the exons of annotated genes. Exons of annotated and unannotated transcripts separated by large genomic distances can be joined together in chimeric transcripts. Any given base-pair in a genome could be traversed by many protein-coding and non-coding RNAs. We discuss the implications of these effects for our understanding of disease.
过去十年收集的大量实验数据表明，基因组组织非常复杂，并强调了当前的基因注释集并没有完全捕捉到这种复杂性。在细胞中检测到的大量 RNA 被发现源自注释基因的外显子之外。由大的基因组距离分隔的注释和未注释转录本的外显子可以在嵌合转录本中连接在一起。基因组中的任何给定碱基对都可能被许多编码蛋白和非编码 RNA 穿过。我们讨论了这些影响对我们理解疾病的意义。
