Gonzalez R, Griparic L, Vargas V, Burgee K, Santacruz P, Anderson R, Schiewe M, Silva F, Patel A
DaVinci Biosciences LLC, 1239 Victoria Street, Costa Mesa, CA 92627, USA.
Biochem Biophys Res Commun. 2009 Aug 7;385(4):570-5. doi: 10.1016/j.bbrc.2009.05.103. Epub 2009 May 29.
Mesenchymal stem cells (MSCs) isolated from several adult human tissues are reported to be a promising tool for regenerative medicine. In order to broaden the array of tools for therapeutic application, we isolated a new population of cells from adult human testis termed gonadal stem cells (GSCs). GSCs express CD105, CD166, CD73, CD90, STRO-1 and lack hematopoietic markers CD34, CD45, and HLA-DR which are characteristic identifiers of MSCs. In addition, GSCs express pluripotent markers Oct4, Nanog, and SSEA-4. GSCs propagated for at least 64 population doublings and exhibited clonogenic capability. GSCs have a broad plasticity and the potential to differentiate into adipogenic, osteogenic, and chondrogenic cells. These studies demonstrate that GSCs are easily obtainable stem cells, have growth kinetics and marker expression similar to MSCs, and differentiate into mesodermal lineage cells. Therefore, GSCs may be a valuable tool for therapeutic applications.
据报道，从几种成人组织中分离出的间充质干细胞（MSC）是再生医学中一种很有前景的工具。为了拓宽治疗应用的工具范围，我们从成人睾丸中分离出了一种新的细胞群体，称为性腺干细胞（GSC）。GSC表达CD105、CD166、CD73、CD90、STRO-1，且缺乏造血标志物CD34、CD45和HLA-DR，这些是MSC的特征性标识符。此外，GSC表达多能性标志物Oct4、Nanog和SSEA-4。GSC传代至少64次群体倍增，并表现出克隆形成能力。GSC具有广泛的可塑性，有分化为脂肪生成细胞、成骨细胞和软骨生成细胞的潜力。这些研究表明，GSC是易于获得的干细胞，具有与MSC相似的生长动力学和标志物表达，并能分化为中胚层谱系细胞。因此，GSC可能是治疗应用中的一种有价值的工具。
