Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, Budapest, Hungary.
Biochem Biophys Res Commun. 2011 Oct 28;414(3):474-80. doi: 10.1016/j.bbrc.2011.09.089. Epub 2011 Sep 24.
Mesenchymal stem cell like (MSCl) cells were generated from human embryonic stem cells (hESC) through embryoid body formation, and isolated by adherence to plastic surface. MSCl cell lines could be propagated without changes in morphological or functional characteristics for more than 15 passages. These cells, as well as their fluorescent protein expressing stable derivatives, efficiently supported the growth of undifferentiated human embryonic stem cells as feeder cells. The MSCl cells did not express the embryonic (Oct4, Nanog, ABCG2, PODXL, or SSEA4), or hematopoietic (CD34, CD45, CD14, CD133, HLA-DR) stem cell markers, while were positive for the characteristic cell surface markers of MSCs (CD44, CD73, CD90, CD105). MSCl cells could be differentiated toward osteogenic, chondrogenic or adipogenic directions and exhibited significant inhibition of mitogen-activated lymphocyte proliferation, and thus presented immunosuppressive features. We suggest that cultured MSCl cells can properly model human MSCs and be applied as efficient feeders in hESC cultures.
间充质干细胞样 (MSCl) 细胞通过胚状体形成从人胚胎干细胞 (hESC) 中产生，并通过黏附于塑料表面进行分离。MSCl 细胞系在超过 15 代的传代过程中形态学或功能特征没有变化，可以进行增殖。这些细胞及其荧光蛋白表达的稳定衍生物作为饲养细胞能够有效地支持未分化的人胚胎干细胞的生长。MSCl 细胞不表达胚胎 (Oct4、Nanog、ABCG2、PODXL 或 SSEA4) 或造血 (CD34、CD45、CD14、CD133、HLA-DR) 干细胞标志物，而对间充质干细胞的特征性细胞表面标志物 (CD44、CD73、CD90、CD105) 呈阳性。MSCl 细胞可向成骨、软骨或成脂方向分化，并表现出对有丝分裂原激活的淋巴细胞增殖的显著抑制作用，因此表现出免疫抑制特征。我们认为，培养的 MSCl 细胞可以很好地模拟人骨髓间充质干细胞，并作为高效的饲养细胞应用于 hESC 培养中。
