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电离辐射对睾丸间质细胞的影响。

The effect of ionizing radiation on testicular interstitial stromal cells.

作者信息

Tamam Esraa, Yan Chen, Sekiya Reiko, Li Tao-Sheng

机构信息

Department of Stem Cell Biology, Atomic Bomb Disease Institute, Graduate School of Biomedical Sciences Nagasaki University Nagasaki Japan.

Department of Anatomy and Embryology, Faculty of Veterinary Medicine Sohag University Sohag Governate Egypt.

出版信息

Reprod Med Biol. 2025 Mar 19;24(1):e12639. doi: 10.1002/rmb2.12639. eCollection 2025 Jan-Dec.

DOI:10.1002/rmb2.12639
PMID:40110174
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11920815/
Abstract

PURPOSE

Testis is one of the most radiosensitive tissues. Interstitial stromal cells play a supportive role in male fertility, but radiation-induced damage to those cells has not yet been well understood. We aimed to investigate radiation-induced changes in interstitial stromal cells in the testis.

METHODS

Adult male C57BL/6N mice (8 weeks) received a single pelvic exposure to a relatively high dose (1 Gy) or a very high dose (8 Gy) X-ray. We collected the testicular tissues for evaluation at 1, 9, and 60 days after irradiation.

RESULTS

We detected a recoverable moderate degeneration of seminiferous tubules after 1 Gy exposure but an irreversible severe damage to the testis after 8 Gy exposure. Immunostaining results revealed that 1 Gy exposure induced DNA damage at day 1, upregulated intratubular GDNF at days 1 and 9, upregulated FGF at all time points, and upregulated CSF-1R at day 9. In contrast, 8 Gy exposure induced DNA damage at days 1 and 9, upregulated intratubular GDNF at days 1 and 9, downregulated CD105 at day 60, and upregulated FGF at all time points.

CONCLUSION

Radiation-induced dynamic changes to interstitial stromal cells in the testis. Upregulated interstitial CSF-1R and FGF2 may support spermatogenesis recovery after high-dose radiation.

摘要

目的

睾丸是对辐射最敏感的组织之一。间质细胞在男性生育中起支持作用，但辐射对这些细胞造成的损伤尚未得到充分了解。我们旨在研究辐射诱导的睾丸间质细胞变化。

方法

成年雄性C57BL/6N小鼠（8周龄）接受单次盆腔相对高剂量（1 Gy）或非常高剂量（8 Gy）的X射线照射。在照射后1、9和60天收集睾丸组织进行评估。

结果

我们检测到1 Gy照射后生精小管有可恢复的中度退化，但8 Gy照射后睾丸有不可逆的严重损伤。免疫染色结果显示，1 Gy照射在第1天诱导DNA损伤，在第1天和第9天上调管内胶质细胞源性神经营养因子（GDNF），在所有时间点上调成纤维细胞生长因子（FGF），并在第9天上调集落刺激因子1受体（CSF-1R）。相比之下，8 Gy照射在第1天和第9天诱导DNA损伤，在第1天和第天上调管内GDNF，在第60天下调CD105，并在所有时间点上调FGF。

结论

辐射诱导睾丸间质细胞发生动态变化。间质CSF-1R和FGF2上调可能支持高剂量辐射后生精功能的恢复。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/4572bf3babf1/RMB2-24-e12639-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/1cd165ac7ca5/RMB2-24-e12639-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/554159ecd403/RMB2-24-e12639-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/fcf5955f4835/RMB2-24-e12639-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/4d49082b117b/RMB2-24-e12639-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/ffb1addf8158/RMB2-24-e12639-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/b202975ba02c/RMB2-24-e12639-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/4572bf3babf1/RMB2-24-e12639-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/1cd165ac7ca5/RMB2-24-e12639-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/554159ecd403/RMB2-24-e12639-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/fcf5955f4835/RMB2-24-e12639-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/4d49082b117b/RMB2-24-e12639-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/ffb1addf8158/RMB2-24-e12639-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/b202975ba02c/RMB2-24-e12639-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64c4/11920815/4572bf3babf1/RMB2-24-e12639-g005.jpg

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本文引用的文献

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Front Reprod Health. 2022 Jul 14;4:904804. doi: 10.3389/frph.2022.904804. eCollection 2022.
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The Fate of Leydig Cells in Men with Spermatogenic Failure.生精功能衰竭男性患者睾丸间质细胞的命运
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Nicaraven mitigates radiation-induced lung injury by downregulating the NF-κB and TGF-β/Smad pathways to suppress the inflammatory response.
尼卡巴嗪通过下调 NF-κB 和 TGF-β/Smad 通路抑制炎症反应，减轻放射性肺损伤。
J Radiat Res. 2022 Mar 17;63(2):158-165. doi: 10.1093/jrr/rrab112.
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The role of endoglin and its soluble form in pathogenesis of preeclampsia.内皮糖蛋白及其可溶性形式在子痫前期发病机制中的作用。
Mol Cell Biochem. 2022 Feb;477(2):479-491. doi: 10.1007/s11010-021-04294-z. Epub 2021 Nov 16.
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