硫醇对淋巴细胞膜的作用。
The action of thiols on lymphocyte membranes.
作者信息
Ryzewski J, Roszkowski-Sliź W, Krzystyniak K
出版信息
Immunology. 1976 Jul;31(1):145-9.
Abstract
Short-term incubation with cysteine in concentrations not altering cell viability increased the amount of -SH groups on the surface of rat lymphocytes. Maximal effect was achieved in 10 min using 2 mM cysteine, which concentration also induced immediate total loss of response to adrenaline, as measured by intracellular cyclic AMP level. Neither of these effects was observed after incubation of lymphocytes with glutathione or dithiothreitol.
摘要
用浓度不改变细胞活力的半胱氨酸进行短期孵育，可增加大鼠淋巴细胞表面的-SH基团数量。使用2 mM半胱氨酸在10分钟内可达到最大效果，该浓度还会导致通过细胞内环磷酸腺苷水平测量的对肾上腺素的反应立即完全丧失。用谷胱甘肽或二硫苏糖醇孵育淋巴细胞后，未观察到这些效应。
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