Juárez Tomás María Silvina, Bru Elena, Martos Gladys, Nader-Macías María Elena
Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, Tucumán, Argentina.
Can J Microbiol. 2009 May;55(5):544-52. doi: 10.1139/w08-159.
The industrial use of lactic acid bacteria as probiotic cultures depends on the preservation techniques employed, which are required to guarantee stable cultures in terms of viability and functional activity. The aim of this study was to evaluate the effects of 12% lactose and 12% sucrose suspended in water or reconstituted skim milk on the survival and expression of beneficial characteristics during freeze-drying and subsequent storage of 6 vaginal lactobacilli strains. A cubic polynomial model was also used for the first time to evaluate the effects of different protectors on survival behavior during storage. Different survival patterns were observed among the strains considered. The presence of both lactose and sucrose in water or in 6% skim milk as the suspension medium proved to be effective in maintaining a high degree of survival and expression of potentially probiotic characteristics (production of antimicrobial substances or auto-aggregation capabilities) of most strains after lyophilization and long-term storage. This study constitutes a valuable step to obtain concentrated cultures with the highest stability of microorganisms for pharmaceutical purposes.
将乳酸菌用作益生菌培养物的工业应用取决于所采用的保存技术，这些技术需要保证培养物在活力和功能活性方面的稳定性。本研究的目的是评估悬浮于水或复原脱脂乳中的12%乳糖和12%蔗糖对6株阴道乳酸杆菌菌株冻干及后续储存期间存活和有益特性表达的影响。还首次使用三次多项式模型来评估不同保护剂对储存期间存活行为的影响。在所研究的菌株中观察到了不同的存活模式。事实证明，以水或6%脱脂乳作为悬浮介质时，乳糖和蔗糖的存在对于大多数菌株在冻干和长期储存后维持高度存活以及潜在益生菌特性（抗菌物质的产生或自聚集能力）的表达是有效的。本研究为获得用于制药目的的具有最高微生物稳定性的浓缩培养物迈出了重要一步。
