Wang Jinsong, Chen Lanming
Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, College of Food Science and Technology, Shanghai Ocean University, 999 Hu Cheng Huan Road, Shanghai 201306, China.
College of Bioengineering, Jingchu University of Technology, 33 Xiang Shan Road, Jingmen 448000, China.
Foods. 2021 Mar 4;10(3):529. doi: 10.3390/foods10030529.
Probiotics are considered as desirable alternatives to antibiotics because of their beneficial effects on the safety and economy of farm animals. The protectant can ensure the viability of probiotics, which is the prerequisite of the beneficial effects. The objective of this study was to evaluate the effects of a novel nano-protectant containing trehalose, skim milk powder, phytoglycogen nanoparticles, and nano-phytoglycogen Pickering emulsions on the viability of K17 under different conditions. The results indicated that the optimal concentration of the carbohydrate substrate was determined to be 10% skim milk powder (/) instead of trehalose. The combination of 10% skim milk powder (/), 1% phytoglycogen nanoparticles (/), and 10% Pickering emulsions (/) was selected as the optimal component of the protectant. Trilayer protectants with an optimal component had a more significant protective effect on the bacteria than that of the monolayer and bilayer protectants, or the control in feed storage, freeze-drying, and simulated gastrointestinal environment. A scanning electron microscope was used to monitor the morphological characteristics of the protectants for different layers on . In conclusion, the trilayer protectant exhibited a substantial effect on during storage and consumption, which could be used in the feed and functional food.
由于益生菌对农场动物的安全性和经济性具有有益影响，因此被认为是抗生素的理想替代品。保护剂可以确保益生菌的活力，这是产生有益效果的前提条件。本研究的目的是评估一种新型纳米保护剂（含有海藻糖、脱脂奶粉、植物糖原纳米颗粒和纳米植物糖原皮克林乳液）在不同条件下对K17活力的影响。结果表明，确定碳水化合物底物的最佳浓度为10%脱脂奶粉（/）而非海藻糖。选择10%脱脂奶粉（/）、1%植物糖原纳米颗粒（/）和10%皮克林乳液（/）的组合作为保护剂的最佳成分。具有最佳成分的三层保护剂对细菌的保护作用比单层和双层保护剂或饲料储存、冷冻干燥和模拟胃肠道环境中的对照更为显著。使用扫描电子显微镜监测不同层保护剂的形态特征。总之，三层保护剂在储存和消费过程中对（此处原文缺失具体所指内容）表现出显著效果，可用于饲料和功能性食品。
