热处理和鱼血清浓度对中国仓鼠卵巢细胞贴壁培养中细胞生长的影响。

Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells.

机构信息

Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo, 060-8628, Japan.

出版信息

Cytotechnology. 2009 Mar;59(2):135-41. doi: 10.1007/s10616-009-9199-7. Epub 2009 May 30.

DOI:10.1007/s10616-009-9199-7

PMID:19484373

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2698441/

Abstract

The effects of heat treatment and concentration of fish serum (FS) on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of recombinant Chinese hamster ovary (CHO) cells, DR1000L4N, were investigated. The addition of heat treated FS instead of non-heat-treated FS improved cell growth in terms of cell density, which reached 60% that in 10% fetal calf serum (FCS)-containing medium (FCS medium). A decrease in FS concentration from 10 to 1.25% markedly increased cell density, which was 79% that in 10% FCS medium. The combination of heat treatment at 56 degrees C and the addition of FS at a low concentration (1.25%) showed an additive effect on cell growth and resulted in the same cell density as that in 10% FCS medium, whereas the hGM-CSF concentration in the culture using FS-containing medium (FS medium) was approximately 50% that in 10% FCS medium. The total lipid concentration in FS was more than three fold that in FCS. The effect of decreasing FS concentration on cell growth may be due to the low lipid concentration in FS medium, because addition of the lipids extracted from FS to 10% FCS and 1.25% FS media markedly decreased cell density. Consequently, the addition of heat-treated FS at low concentrations to medium may be useful for the growth of CHO cells without FCS.

摘要

热处理和鱼血清（FS）浓度对重组中国仓鼠卵巢（CHO）细胞系 DR1000L4N 贴壁培养中细胞生长和人粒细胞-巨噬细胞集落刺激因子（hGM-CSF）生产的影响进行了研究。添加热处理 FS 而不是未经热处理的 FS 可改善细胞生长，表现在细胞密度方面，达到含 10%胎牛血清（FCS）培养基（FCS 培养基）的 60%。FS 浓度从 10%降至 1.25%可显著增加细胞密度，达到 10% FCS 培养基的 79%。56°C 热处理与低浓度 FS（1.25%）的组合对细胞生长具有相加作用，导致与 10% FCS 培养基相同的细胞密度，而含有 FS 的培养基（FS 培养基）中 hGM-CSF 浓度约为 10% FCS 培养基的 50%。FS 中的总脂质浓度是 FCS 的三倍多。FS 浓度降低对细胞生长的影响可能是由于 FS 培养基中的脂质浓度较低，因为将从 FS 中提取的脂质添加到 10% FCS 和 1.25% FS 培养基中会显著降低细胞密度。因此，向培养基中添加低浓度的热处理 FS 可能有助于在没有 FCS 的情况下 CHO 细胞的生长。