用于单分子测量的微流控混合系统。

A microfluidic mixing system for single-molecule measurements.

作者信息

Pfeil Shawn H, Wickersham Charles E, Hoffmann Armin, Lipman Everett A

机构信息

Department of Physics, University of California, Santa Barbara, California 93106, USA.

出版信息

Rev Sci Instrum. 2009 May;80(5):055105. doi: 10.1063/1.3125643.

DOI:10.1063/1.3125643

PMID:19485532

Abstract

This article describes the design and fabrication of a microfluidic mixing system optimized for ultrasensitive optical measurements. Channels are replica-molded in polydimethylsiloxane elastomer and sealed with fused-silica coverglass. The resulting devices have broad chemical compatibility and extremely low fluorescence background, enabling measurements of individual molecules under well-characterized nonequilibrium conditions. Fluid delivery and pressure connections are made using an interface that allows for rapid assembly, rapid sample exchange, and modular device replacement while providing access for high numerical aperture optics.

摘要

本文介绍了一种针对超灵敏光学测量进行优化的微流控混合系统的设计与制造。通道通过复制模塑法在聚二甲基硅氧烷弹性体中成型，并用熔融石英盖玻片密封。所得器件具有广泛的化学兼容性和极低的荧光背景，能够在特征明确的非平衡条件下对单个分子进行测量。流体输送和压力连接通过一个接口实现，该接口允许快速组装、快速样品交换和模块化设备更换，同时为高数值孔径光学元件提供接入通道。