Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany.
Appl Microbiol Biotechnol. 2012 Mar;93(5):2205-14. doi: 10.1007/s00253-011-3543-5. Epub 2011 Aug 26.
A strain Rhodococcus sp. OCT 10 DSM 45596(T), exhibiting 99.9% of 16S rDNA identity with Rhodococcus wratislaviensis NCIMB 13082, was isolated from a soil sample. The strain completely mineralised 2-chlorotoluene, 2-bromotoluene, o-xylene, benzyl alcohol and benzoate. In contrast, 2-fluorotoluene was only partially mineralised. By GC-MS and (1)H-NMR analyses, 4-chloro-3-methylcatechol was identified as the central intermediate in the degradation pathway of 2-chlorotoluene. It was further degraded by enzymes of the meta cleavage pathway. Catechol 1,2-dioxygenase and chlorocatechol 1,2-dioxygenase as the initial enzymes of the ortho cleavage pathways were not detectable under these conditions. Furthermore, neither formation nor oxidation of 2-chlorobenzylic alcohol, 2-chlorobenzaldehyde, or 2-chlorobenzoate was observed, thereby excluding side chain oxidation activity.
一株 Rhodococcus sp. OCT 10 DSM 45596(T)，其 16S rDNA 序列与 Rhodococcus wratislaviensis NCIMB 13082 的同源性达到 99.9%，从土壤样本中分离得到。该菌株能完全矿化 2-氯甲苯、2-溴甲苯、邻二甲苯、苯甲醇和苯甲酸。相比之下，2-氟甲苯只能部分矿化。通过 GC-MS 和 (1)H-NMR 分析，确定 4-氯-3-甲基儿茶酚是 2-氯甲苯降解途径中的中心中间产物。它进一步通过间位裂解途径的酶降解。邻位裂解途径的初始酶儿茶酚 1,2-双加氧酶和氯儿茶酚 1,2-双加氧酶在这些条件下无法检测到。此外，既没有观察到 2-氯苄醇、2-氯苯甲醛或 2-氯苯甲酸的形成或氧化，从而排除了侧链氧化活性。
