Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
J Appl Microbiol. 2009 Oct;107(4):1251-7. doi: 10.1111/j.1365-2672.2009.04304.x. Epub 2009 Apr 9.
To investigate the species-specific prevalence of vhhP2 among Vibrio harveyi isolates and the applicability of vhhP2 in the specific detection of V. harveyi from crude samples of animal and environmental origins.
A gene (vhhP2) encoding an outer membrane protein of unknown function was identified from a pathogenic V. harveyi isolate. vhhP2 is present in 24 V. harveyi strains isolated from different geographical locations but is absent in 24 strains representing 17 different non-V. harveyi species, including V. parahaemolyticus and V. alginolyticus. A simple polymerase chain reaction method for the identification of V. harveyi was developed based on the conserved sequence of vhhP2. This method was demonstrated to be applicable to the quick detection of V. harveyi from crude animal specimens and environmental samples. The specificity of this method was tested by applying it to the examination of two strains of V. campbellii, which is most closely related to V. harveyi. One of the V. campbellii strains was falsely identified as V. harveyi.
vhhP2 is ubiquitously present in the V. harveyi species and is absent in most of the non-V. harveyi species; this feature enables vhhP2 to serve as a genetic marker for the rapid identification of V. harveyi. However, this method can not distinguish some V. campbellii strains from V. harveyi.
the significance of our study is the identification of a novel gene of V. harveyi and the development of a simple method for the relatively accurate detection of V. harveyi from animal specimens and environmental samples.
调查哈维弧菌分离株中外膜蛋白 vhhP2 的种特异性流行情况，以及 vhhP2 在特定检测动物和环境来源粗样中哈维弧菌的适用性。
从一株致病性哈维弧菌分离株中鉴定出一个编码一种未知功能的外膜蛋白的基因（vhhP2）。vhhP2 存在于 24 株来自不同地理位置的哈维弧菌菌株中，但不存在于 24 株代表 17 种不同非哈维弧菌的菌株中，包括副溶血弧菌和溶藻弧菌。基于 vhhP2 的保守序列，开发了一种用于鉴定哈维弧菌的简单聚合酶链反应方法。该方法已被证明适用于从粗动物标本和环境样本中快速检测哈维弧菌。该方法的特异性通过应用于与哈维弧菌最密切相关的两株坎贝尔弧菌菌株的检查来测试。其中一株坎贝尔弧菌菌株被错误地鉴定为哈维弧菌。
vhhP2 在哈维弧菌种中普遍存在，而在大多数非哈维弧菌种中不存在；这一特征使 vhhP2 能够作为快速鉴定哈维弧菌的遗传标记。然而，该方法不能区分某些坎贝尔弧菌菌株与哈维弧菌。
我们研究的意义在于鉴定了哈维弧菌的一种新基因，并开发了一种简单的方法，用于从动物标本和环境样本中相对准确地检测哈维弧菌。
