Patil Shrikant L, Swaroop K, Kakde Nilesh, Somashekarappa H M
Department of Physiology, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India.
Centre for Application of Radioisotopes and Radiation Technology, USIC, Mangalore University, Mangalore, Karnataka, India.
Indian J Nucl Med. 2017 Oct-Dec;32(4):289-295. doi: 10.4103/ijnm.IJNM_30_17.
Rutin (RUT) and quercetin (QRT) which are dietary compounds were investigated for their ability to protect against ionizing radiation (IR)-induced genotoxicity in human lymphocytes.
The radiation antagonistic potential of RUT and QRT was assessed by alkaline comet and cytokinesis-block micronucleus (CBMN) assay.
Treatment of lymphocytes with RUT and QRT (25 μg/ml) prior exposure to 2 Gy gamma radiation resulted in a significant reduction of frequency of micronuclei as compared to the control set of cells evaluated by CBMN assay. Similarly, treatment of lymphocytes with RUT and QRT before radiation exposure showed significant decrease in the DNA damage as assessed by comet parameters, such as percent tail DNA and olive tail moment.
The study demonstrates the protective effect of RUT and QRT against IR-induced DNA damage in human lymphocytes, which may be partly attributed to scavenging of IR-induced free radicals and also by the inhibition of IR-induced oxidative stress.
研究膳食化合物芦丁（RUT）和槲皮素（QRT）对电离辐射（IR）诱导的人类淋巴细胞遗传毒性的防护能力。
通过碱性彗星试验和胞质分裂阻滞微核（CBMN）试验评估RUT和QRT的辐射拮抗潜力。
与通过CBMN试验评估的对照组细胞相比，在暴露于2 Gyγ辐射之前用RUT和QRT（25μg/ml）处理淋巴细胞，导致微核频率显著降低。同样，在辐射暴露前用RUT和QRT处理淋巴细胞，通过彗星试验参数（如尾部DNA百分比和橄榄尾矩）评估，显示DNA损伤显著减少。
该研究证明了RUT和QRT对IR诱导的人类淋巴细胞DNA损伤具有保护作用，这可能部分归因于清除IR诱导的自由基以及抑制IR诱导的氧化应激。
