Institute of Food Safety and Environmental Monitoring, Fuzhou University, P. R. China.
J Sep Sci. 2010 Jul;33(14):2123-30. doi: 10.1002/jssc.200900855.
Molecularly imprinted polymer (MIP) monoliths with (S)-ornidazole ((S)-ONZ) as the template molecule have been designed and prepared by the simple thermal polymerization of methacrylic acid, 4-vinylpyridine, and ethylene dimethacrylate in the presence of a binary porogenic mixture of toluene and dodecanol. The influences of polymerization mixture composition on the chiral recognition of ONZ have been evaluated, and the imprint effect in the optimized MIP monolith has been clearly demonstrated. The new monolithic stationary phase with optimized porous property and good selectivity was used for the chiral separation of ONZ by pressurized CEC. The pressurized CEC conditions were also optimized to obtain the good chiral separation. The enantiomers were rapidly separated within 9 min on the MIP-based chiral stationary phase, whereas the chiral separation was not obtained on the nonimprinted polymer. Additionally, the proposed method has been successfully applied to the chiral separation of ONZ in tablet samples by injection of the crude sample. The cross-selectivity for similar antiparasitic drug was investigated. The results indicated that the chiral separation of secnidazole could also be obtained on the optimized MIP monolith within 14 min.
以（S）-奥硝唑（（S）-ONZ）为模板分子的分子印迹聚合物（MIP）整体柱已通过在甲苯和十二醇二元致孔剂混合物存在下热聚合甲基丙烯酸、4-乙烯基吡啶和乙二醇二甲基丙烯酸酯而设计和制备。研究了聚合混合物组成对 ONZ 手性识别的影响，并在优化的 MIP 整体柱中清楚地证明了印迹效应。具有优化的多孔性和良好选择性的新型整体固定相用于加压毛细管电泳（CEC）对手性奥硝唑的分离。还优化了加压 CEC 条件以获得良好的手性分离。在基于 MIP 的手性固定相上，对映异构体在 9 分钟内迅速分离，而在非印迹聚合物上则未获得手性分离。此外，该方法已成功应用于通过注入粗样品对手表样品中奥硝唑的手性分离。研究了类似抗寄生虫药物的交叉选择性。结果表明，在优化的 MIP 整体柱上也可以在 14 分钟内获得 secnidazole 的手性分离。
