Ward Ryan J, Lee Lilian, Graham Kevin, Satkunendran Thevagi, Yoshikawa Koichi, Ling Erick, Harper Lauren, Austin Ryan, Nieuwenhuis Erica, Clarke Ian D, Hui Chi-Chung, Dirks Peter B
Developmental and Stem Cell Biology, Hospital for Sick Children, ON, Canada M5G 1X8.
Cancer Res. 2009 Jun 1;69(11):4682-90. doi: 10.1158/0008-5472.CAN-09-0342.
Subpopulations of tumorigenic cells have been identified in many human tumors, although these cells may not be very rare in some types of cancer. Here, we report that medulloblastomas arising from Patched-1-deficient mice contain a subpopulation of cells that show a neural precursor phenotype, clonogenic and multilineage differentiation capacity, activated Hedgehog signaling, wild-type Patched-1 expression, and the ability to initiate tumors following allogeneic orthotopic transplantation. The normal neural stem cell surface antigen CD15 enriches for the in vitro proliferative and in vivo tumorigenic potential from uncultured medulloblastomas, supporting the existence of a cancer stem cell hierarchy in this clinically relevant mouse model of cancer.
在许多人类肿瘤中已鉴定出致瘤细胞亚群，尽管这些细胞在某些类型的癌症中可能并不十分罕见。在此，我们报告，源自Patched-1缺陷小鼠的髓母细胞瘤含有一群细胞亚群，这些细胞表现出神经前体细胞表型、克隆形成和多谱系分化能力、激活的Hedgehog信号传导、野生型Patched-1表达以及在同种异体原位移植后引发肿瘤的能力。正常神经干细胞表面抗原CD15增强了未培养髓母细胞瘤的体外增殖和体内致瘤潜力，支持了这种临床相关癌症小鼠模型中癌症干细胞层级的存在。
