Shilov E M, Gabrilevskaia O V, Ivanov A A, Gladskikh O P, Leskov V P, Pal'tsev M A, Tareeva I E
Ter Arkh. 1991;63(6):21-6.
To specify the role of mononuclear (MN) leukocytes in the development of mesangial cell proliferation, which is one of the main manifestations of glomerulonephritis, cell cultures of renal glomeruli of rats with nephrotoxic serum (NTS) nephritis were examined for the intensity of mesangial cell proliferation, the MN-leukocyte count, and for IL-1 production. The amount of mesangial cells and the intensity of their proliferation in the cultures of glomerular cells from rats with NTS nephritis were much higher than in intact rat cultures. At the same time the glomerular cultures from rats with NTS nephritis demonstrated an increase in the MN leukocyte count together with enhancement of IL-1 production. The treatment with prednisolone averted accumulation of MN leukocytes by the glomerular cultures and noticeably reduced mesangial cell proliferation. The supernatant liquid of cultures of peripheral blood MN leukocytes from patients with active nephritis suppressed human fibroblast proliferation, exerting no such action on mesangial cell culture. During the treatment with prednisolone, the supernatant liquid produced a reverse effect on fibroblast and mesangial cell cultures, which was associated with the clinical improvement of the health status. It is assumed that mesangial cell proliferation seen in nephritis may be related to infiltration of the glomeruli by MN leukocytes and to elevated production by them of IL-1 and that the therapeutic action of prednisolone may be determined by suppression of these processes.
为明确单核（MN）白细胞在肾小球肾炎主要表现之一的系膜细胞增殖发展过程中的作用，对患有肾毒性血清（NTS）肾炎大鼠的肾小球进行细胞培养，检测系膜细胞增殖强度、MN白细胞计数及白细胞介素-1（IL-1）的产生情况。NTS肾炎大鼠肾小球细胞培养物中系膜细胞数量及其增殖强度远高于正常大鼠培养物。同时，NTS肾炎大鼠的肾小球培养物显示MN白细胞计数增加，且IL-1产生增强。泼尼松龙治疗可避免肾小球培养物中MN白细胞的积聚，并显著减少系膜细胞增殖。活动性肾炎患者外周血MN白细胞培养物的上清液可抑制人成纤维细胞增殖，但对系膜细胞培养无此作用。在泼尼松龙治疗期间，上清液对成纤维细胞和系膜细胞培养物产生相反作用，这与健康状况的临床改善相关。据推测，肾炎中所见的系膜细胞增殖可能与MN白细胞浸润肾小球及其IL-1产生增加有关，且泼尼松龙的治疗作用可能由对这些过程的抑制所决定。
