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电压钳对细菌趋化性的影响。

Voltage clamp effects on bacterial chemotaxis.

作者信息

Margolin Y, Eisenbach M

出版信息

J Bacteriol. 1984 Aug;159(2):605-10. doi: 10.1128/jb.159.2.605-610.1984.

Abstract

To examine whether or not sensory signaling in bacteria is by way of fluctuations in membrane potential, we studied the effect of clamping the potential on bacterial chemotaxis. The potential was clamped by valinomycin, a K+ -specific ionophore, in the presence of K+. Despite the clamped potential, sensory signaling did occur: both Escherichia coli and Bacillus subtilis cells were still excitable and adaptable under these conditions. It is concluded that signaling in the excitation and adaptation steps of chemotaxis is not by way of fluctuations in the membrane potential.

摘要

为了研究细菌中的感觉信号传导是否通过膜电位波动进行,我们研究了钳制电位对细菌趋化性的影响。在钾离子存在的情况下,通过缬氨霉素(一种钾离子特异性离子载体)钳制电位。尽管电位被钳制,但感觉信号传导仍会发生:在这些条件下,大肠杆菌和枯草芽孢杆菌细胞仍然具有兴奋性和适应性。得出的结论是,趋化性的兴奋和适应步骤中的信号传导不是通过膜电位波动进行的。

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