N Mithun, Lukose Jijo, Shastry Shamee, Mohan Ganesh, Chidangil Santhosh
Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education Karnataka 576104 India
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal, Manipal Academy of Higher Education Manipal Karnataka 576104 India.
RSC Adv. 2020 Aug 26;10(52):31453-31462. doi: 10.1039/d0ra05842d. eCollection 2020 Aug 21.
Hydroxyethyl starch (HES) is a commonly used intravenous fluid in hospital settings. The merits and demerits of its application is still a debatable topic. Investigating the interaction of external agents like intravenous fluids with blood cells is of great significance in clinical environments. Micro-Raman spectroscopy combined with an optical tweezers technique has been utilized for conducting systematic investigations of single live red blood cells (RBCs) under the influence of external stress agents. The present work deals with a detailed biophysical study on the response of human live red blood cells in hydroxyethyl starch using optical techniques. Morphological changes in red blood cells were monitored using quantitate phase imaging techniques. Micro-Raman studies suggest that there is a significant reduction in the oxy-haemoglobin level in red blood cells suspended in HES. The spectra recorded by using different probe laser powers has shown that the cells are more vulnerable in HES under the influence of externally induced stress than in blood plasma. In addition, the spectral results support the possibility of heme aggregation and membrane damage for red blood cells in HES under externally induced stress. Principle component analysis performed on the Raman spectra were able to effectively discriminate between red blood cells in HES and in blood plasma. The use of Raman tweezers can be highly beneficial in elucidating biochemical alterations happening in live, human red blood cell.
羟乙基淀粉（HES）是医院环境中常用的静脉输液。其应用的优缺点仍是一个有争议的话题。在临床环境中，研究诸如静脉输液等外部因素与血细胞的相互作用具有重要意义。显微拉曼光谱结合光镊技术已被用于对外部应激因素影响下的单个活红细胞（RBC）进行系统研究。目前的工作涉及使用光学技术对人活红细胞在羟乙基淀粉中的反应进行详细的生物物理研究。使用定量相成像技术监测红细胞的形态变化。显微拉曼研究表明，悬浮在HES中的红细胞中氧合血红蛋白水平显著降低。使用不同探测激光功率记录的光谱表明，在外部诱导应激的影响下，细胞在HES中比在血浆中更易受损。此外，光谱结果支持在外部诱导应激下HES中的红细胞发生血红素聚集和膜损伤的可能性。对拉曼光谱进行的主成分分析能够有效区分HES中和血浆中的红细胞。拉曼镊子的使用在阐明活的人红细胞中发生的生化变化方面可能非常有益。
