Willander M, Al-Hilli Safaa
Department of Science and Technology, Linköping University, Gothenburg University, Norrköpin, Sweden.
Methods Mol Biol. 2009;544:187-200. doi: 10.1007/978-1-59745-483-4_13.
High-density ZnO nanorods of 60-80 nm in diameter and 500-700 nm in length grown on the silver-coated tip of a borosilicate glass capillary (0.7 mum in diameter) demonstrate a remarkable linear response to proton H(3)O(+) concentrations in solution. These nanorods were used to create a highly sensitive pH sensor for monitoring in vivo biological process within single cells. The ZnO nanorods exhibit a pH-dependent electrochemical potential difference versus an Ag/AgCl microelectrode. The potential difference was linear over a large dynamic range (pH, 4-11) and had a sensitivity equal to 51.88 mV/pH at 22 degrees C, which could be understood in terms of changes in surface charge during protonation and deprotonation. Vertically grown nanoelectrodes of this type can be smoothly and gently applied to penetrate a single living cell without causing cell apoptosis.
在直径为0.7微米的硼硅酸盐玻璃毛细管的镀银尖端上生长的直径为60 - 80纳米、长度为500 - 700纳米的高密度氧化锌纳米棒，对溶液中的质子H₃O⁺浓度表现出显著的线性响应。这些纳米棒被用于制造一种高度灵敏的pH传感器，用于监测单细胞内的体内生物过程。氧化锌纳米棒相对于Ag/AgCl微电极表现出pH依赖的电化学势差。该势差在较大的动态范围（pH值为4 - 11）内呈线性，在22℃时灵敏度为51.88 mV/pH，这可以通过质子化和去质子化过程中表面电荷的变化来理解。这种垂直生长的纳米电极可以平稳、轻柔地应用于穿透单个活细胞而不会导致细胞凋亡。
