Department of Science and Technology, Campus Norrköping, Linköping University, Bredgatan 34, SE-601 74 Norrköping, Sweden.
Biosens Bioelectron. 2010 Jun 15;25(10):2205-11. doi: 10.1016/j.bios.2010.02.025. Epub 2010 Mar 3.
In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 microm diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 microM). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration.
在本文中，我们报道了一种基于功能化 ZnO 纳米棒的选择性电化学传感器，用于检测细胞内葡萄糖。为了调整传感器以用于细胞内葡萄糖测量，我们在银覆盖的硼硅酸盐玻璃毛细管（0.7 微米直径）的尖端生长了六方 ZnO 纳米棒，并在其上涂覆了葡萄糖氧化酶。涂有酶的 ZnO 纳米棒表现出与 Ag/AgCl 参比微电极的葡萄糖依赖性电化学电势差。在感兴趣的浓度范围内（0.5-1000μM），电势差呈线性关系。使用我们的 ZnO 纳米棒传感器测量人脂肪细胞或蛙卵母细胞中的葡萄糖浓度与文献中报道的葡萄糖浓度值一致；此外，该传感器能够表明胰岛素增加了细胞内葡萄糖浓度。这种纳米电极装置展示了一种简单的技术，可用于测量细胞内葡萄糖浓度。
