John Innes Centre, Norwich, NR4 7UH, UK.
IBERS, Aberystwyth University, Plas Gogerddan, Aberystwyth, SY23 3EE, UK.
New Phytol. 2018 Oct;220(1):288-299. doi: 10.1111/nph.15286. Epub 2018 Jul 5.
Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown. Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing. We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule. Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.
豌豆（Pisum sativum）是相对少数几种具有复叶的遗传上易于处理的植物物种之一。豌豆叶具有各种特化器官：小叶、卷须、叶枕和托叶，这使得能够鉴定出改变或影响叶片不同部分的突变。这些突变的特征提供了对新型叶片特征的发育和进化的深入了解。先前表征的形态学基因 Cochleata 赋予托叶身份，已知与 Stipules reduced (St) 相互作用，后者调节豌豆托叶的大小，但 St 基因仍然未知。在这里，我们通过限制性位点相关 DNA 测序分析了快中子辐照豌豆突变体。我们鉴定出 St 是一个编码 C2H2 锌指转录因子的基因，该基因受 Cochleata 调控。St 调节托叶中的细胞分裂和细胞扩张。我们的方法表明，如何成功地对全基因组序列不可用的物种的性状进行系统的全基因组筛选分析。
