Lin Hai-Shu, Yue Bing-De, Ho Paul C
Department of Pharmacy, National University of Singapore, Singapore.
Biomed Chromatogr. 2009 Dec;23(12):1308-15. doi: 10.1002/bmc.1254.
A simple HPLC-UV method was developed and validated for the quantification of pterostilbene (3,5-dimethoxy-4'-hydroxy-trans-stilbene), a pharmacologically active phytoalexin in rat plasma. The assay was carried out by measuring the UV absorbance at 320 nm. Pterostilbene and the internal standard, 3,5,4'-trimethoxy-trans-stilbene eluted at 5.7 and 9.2 min, respectively. The calibration curve (20-2000 ng/mL) was linear (R(2)> 0.997). The lower limits of detection and of quantification were 6.7 and 20 ng/mL, respectively. The intra- and inter-day precisions in terms of RSD were all lower than 6%. The analytical recovery ranged from 95.5 +/- 3.7 to 103.2 +/- 0.7% while the absolute recovery ranged from 101.9 +/- 1.1 to 104.9 +/- 4.4%. This simple HPLC method was subsequently applied in a pharmacokinetic study carried out in Sprague-Dawley rats. The terminal elimination half-life and clearance of pterostilbene were 96.6 +/- 23.7 min and 37.0 +/- 2.5 mL/min/kg, respectively, while its absolute oral bioavailability was 12.5 +/- 4.7%. Pterostilbene appeared to have better pharmacokinetic characteristics than its natural occurring analog, resveratrol.
开发并验证了一种简单的高效液相色谱 - 紫外（HPLC - UV）方法，用于定量测定大鼠血浆中具有药理活性的植物抗毒素紫檀芪（3,5 - 二甲氧基 - 4'- 羟基 - 反式芪）。该测定通过测量320 nm处的紫外吸光度进行。紫檀芪和内标3,5,4'- 三甲氧基 - 反式芪分别在5.7分钟和9.2分钟洗脱。校准曲线（20 - 2000 ng/mL）呈线性（R(2)> 0.997）。检测限和定量下限分别为6.7 ng/mL和20 ng/mL。日内和日间精密度的相对标准偏差（RSD）均低于6%。分析回收率在95.5 +/- 3.7%至103.2 +/- 0.7%之间，而绝对回收率在101.9 +/- 1.1%至104.9 +/- 4.4%之间。这种简单的HPLC方法随后应用于在Sprague - Dawley大鼠中进行的药代动力学研究。紫檀芪的末端消除半衰期和清除率分别为96.6 +/- 23.7分钟和37.0 +/- 2.5 mL/min/kg，而其绝对口服生物利用度为12.5 +/- 4.7%。紫檀芪似乎比其天然类似物白藜芦醇具有更好的药代动力学特征。
