Inagaki Shinsuke, Makino Hatsune, Fukushima Takeshi, Min Jun Zhe, Toyo'oka Toshimasa
Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, and Global COE Program, University of Shizuoka, Surugaku, Shizuoka, Japan.
Biomed Chromatogr. 2009 Dec;23(12):1245-50. doi: 10.1002/bmc.1256.
A new method for the rapid and simultaneous detection of ketamine and its major metabolite, norketamine, in rat hair has been developed by combining micropulverized extraction and ultraperformance liquid chromatography-electrospray ionization mass spectrometry. By using reversed-phase UPLC, ketamine and norketamine were well separated within 2 min. Using ketamine-dosed rat hair, the conditions for micropulverized extraction were optimized, and the limits of detection and quantification of the developed method were found to be 1.7 and 5.7 pg/mg hair for ketamine, respectively. The precisions achieved with this method were slightly better than that obtained with conventional acidic methanol extraction method. Using this proposed method, analysis of the washed rat hair could be completed within 16-17 min. This method is expected to be applied for the analysis of the hair samples of not only rats but also ketamine abusers.
通过将微粉化萃取与超高效液相色谱-电喷雾电离质谱联用，开发了一种快速同时检测大鼠毛发中氯胺酮及其主要代谢物去甲氯胺酮的新方法。采用反相超高效液相色谱法，氯胺酮和去甲氯胺酮在2分钟内得到良好分离。以染毒氯胺酮的大鼠毛发为样本，对微粉化萃取条件进行了优化，所建立方法对氯胺酮的检测限和定量限分别为1.7 pg/mg毛发和5.7 pg/mg毛发。该方法的精密度略优于传统酸性甲醇萃取法。使用该方法，清洗后的大鼠毛发分析可在16 - 17分钟内完成。该方法有望应用于大鼠毛发样本分析，也可用于氯胺酮滥用者毛发样本分析。
