Molecular systematics of the three mitochondrial protein-coding genes of malaria parasites: corroborative and new evidence for the origins of human malaria.

BACKGROUND AND AIMS

The mitochondrial genomes of malaria parasites (Plasmodium and related genera) are extremely small and contain just three protein-coding genes. These short linear genomes are tandemly repeated, allowing for amplification of the entire unit using a single pair of outwardly facing primers.

MATERIALS AND METHODS

Using this approach, I sequenced full mitochondrial genomes for seven new lineages of these parasites belonging to four genera and then, combining these new sequences with other published ones, I examined the phylogenetic utility of each of the three protein-coding genes, alone and when concatenated into a data-set of 3315 nucleotides.

RESULTS

Most relationships recovered are consistent with previous studies of the group.

CONCLUSION

Support for an Asian origin of Plasmodium vivax and a sister relationship of Plasmodium falciparum to the rodent malaria parasites was observed in this study. However, if a broad understanding of the evolutionary relationships of this group is to truly be understood, it is clear that loci outside the mitochondrial genome should be explored.