Ayaki Masahiko, Noda Yoshiko, Yaguchi Shigeo, Koide Ryohei, Iwasawa Atsuo, Inoue Toshu, Inoue Yoichi
Department of Ophthalmology, Fujigaoka Hospital, Showa University School of Medicine, Yokohama, Japan.
Nippon Ganka Gakkai Zasshi. 2009 May;113(5):576-82.
The cytotoxicity of a range of commercial antiglaucoma ophthalmic solutions was assessed in human corneal endothelial cells using in vitro techniques.
Cell survival was measured using the WST-1 assay for endothelial cells and the MTT assay for epithelial cells. Commercially available timolol, carteolol, latanoplast, unoprostone, levobunolol, bunazosine, betaxolol, nipradiol, dorzolamide, brinzolamide, and pilocarpine were assessed. The survival of cells exposed to test ophthalmic solutions was expressed as a percentage of cell survival in the control solution (distilled water added to media) after 48 hours exposure.
Survival was lower in prostagrandines and in medications containing benzalkonium. It increased to more than 85% after dilution of 1000-fold or more dilution.
Antiglaucoma ophthalmic solutions have corneal endothelial toxicity. The toxicity significantly decreases after dilution of 1000-fold or more dilution and toxicity seems to be due mostly to benzalkonium chloride.
采用体外技术评估一系列市售抗青光眼眼药水对人角膜内皮细胞的细胞毒性。
使用WST - 1法检测内皮细胞的细胞存活率，使用MTT法检测上皮细胞的细胞存活率。对市售的噻吗洛尔、卡替洛尔、拉坦前列素、乌诺前列酮、左布诺洛尔、布那唑嗪、倍他洛尔、尼普地洛、多佐胺、布林佐胺和毛果芸香碱进行了评估。将暴露于测试眼药水的细胞存活率表示为在48小时暴露后对照溶液（添加到培养基中的蒸馏水）中细胞存活率的百分比。
前列腺素类药物和含苯扎氯铵的药物中细胞存活率较低。在稀释1000倍或更高倍数后，细胞存活率增加到85%以上。
抗青光眼眼药水具有角膜内皮毒性。在稀释1000倍或更高倍数后，毒性显著降低，毒性似乎主要归因于苯扎氯铵。
