Jagusztyn-Krynicka Elzbieta Katarzyna, Dadlez Michał, Grabowska Anna, Roszczenko Paula
Department of Bacterial Genetics, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Expert Rev Proteomics. 2009 Jun;6(3):315-30. doi: 10.1586/epr.09.47.
Infectious diseases still remain the main cause of human premature deaths, especially in developing countries. Vaccines constitute the most cost-effective tool for prophylaxis of infectious diseases. Elucidation of the complete genomes of many bacterial pathogens has provided a new blueprint for the search of novel vaccine candidates. At the same time, it was a turning point in the development of transcriptomics and proteomics. This article concentrates on the proteomic contribution to vaccinology, pointing out relationships between genomic, transcriptomic and proteomic approaches and describing how they complement one another. It also highlights the recent proteomic techniques applied to antigen identification, their capabilities and limitations, as well as the strategies that are taken to overcome technical difficulties and to refine applied methods. Finally, some recent experimental data concerning the proteomic/immunoproteomic influence on identification of vaccine candidates to prevent human infections caused by Streptococcus spp., as well as by a major bioterrorist agent, Bacillus anthracis is presented.
传染病仍然是人类过早死亡的主要原因，尤其是在发展中国家。疫苗是预防传染病最具成本效益的工具。许多细菌病原体完整基因组的阐明为寻找新型候选疫苗提供了新的蓝图。与此同时，这也是转录组学和蛋白质组学发展的一个转折点。本文着重阐述蛋白质组学对疫苗学的贡献，指出基因组学、转录组学和蛋白质组学方法之间的关系，并描述它们如何相互补充。还强调了最近应用于抗原鉴定的蛋白质组学技术、其能力和局限性，以及为克服技术困难和完善应用方法所采取的策略。最后，展示了一些近期关于蛋白质组学/免疫蛋白质组学对鉴定预防由链球菌属以及主要生物恐怖主义病原体炭疽芽孢杆菌引起的人类感染的候选疫苗的影响的实验数据。
