Serruto Davide, Serino Laura, Masignani Vega, Pizza Mariagrazia
Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy.
Vaccine. 2009 May 26;27(25-26):3245-50. doi: 10.1016/j.vaccine.2009.01.072. Epub 2009 Feb 5.
Bacterial infectious diseases remain the single most important threat to health worldwide. Although conventional vaccinology approaches were successful in conferring protection against several diseases, they failed to provide efficacious solutions against many others. The advent of whole-genome sequencing changed the way to think about vaccine development, enabling the targeting of possible vaccine candidates starting from the genomic information of a single bacterial isolate, with a process named reverse vaccinology. As the genomic era progressed, reverse vaccinology has evolved with a pan-genome approach and multi-strain genome analysis became fundamental for the design of universal vaccines. This review describes the applications of genome-based approaches in the development of new vaccines against bacterial pathogens.
细菌感染性疾病仍然是全球健康面临的最重要单一威胁。尽管传统疫苗学方法成功地为多种疾病提供了保护，但它们未能为许多其他疾病提供有效的解决方案。全基因组测序的出现改变了疫苗开发的思路，通过一种名为反向疫苗学的过程，能够从单个细菌分离株的基因组信息出发，确定可能的疫苗候选物。随着基因组时代的发展，反向疫苗学已演变为泛基因组方法，多菌株基因组分析成为通用疫苗设计的基础。本综述描述了基于基因组的方法在开发针对细菌病原体的新型疫苗中的应用。
