Cowley J A, Coman G J, Salmon M L, Young N D, Rajendran K V, Wilson K J, Preston N P
CSIRO Food Futures National Research Flagship, CSIRO Livestock Industries, St Lucia, Queensland, Australia.
Aust Vet J. 2009 Jun;87(6):244-8. doi: 10.1111/j.1751-0813.2009.00436.x.
To identify whether black tiger prawns (Penaeus monodon) in the Weipa region of the Gulf of Carpentaria, Queensland, are free of gill-associated virus (GAV) and Mourilyan virus (MoV), which are endemic in P. monodon along the east coast of Queensland.
Preliminary screening suggested that Weipa might be a source of P. monodon that are free of GAV and MoV. To assess this, more than 150 prawns captured near Weipa were maintained locally in tanks for 2 weeks and bled three times as a stressor to promote higher-level infections. The existence of GAV and MoV in lymphoid organ tissue was then determined using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Some prawns were maintained in tanks for an additional 75 days before being tested.
Real-time qRT-PCR did not detect GAV in any of 33 pools of RNA isolated from the 166 prawns tested. MoV was detected in five pools of RNA at extremely low viral RNA copy numbers close to the sensitivity threshold of the test. MoV was also detected at a similar low copy number in one of nine pearl-oyster mantle samples used as negative controls.
GAV infection is either absent or, like MoV, potentially present at a very low prevalence in juvenile P. monodon inhabiting the inshore waters at Weipa. This region can thus be recommended as a good source of P. monodon certifiable as specific pathogen-free for GAV and MoV, which is desirable for domestication and selective breeding programs in Australia.
确定昆士兰州卡奔塔利亚湾韦帕地区的黑虎虾（斑节对虾）是否未感染鳃相关病毒（GAV）和莫里林病毒（MoV），这两种病毒在昆士兰州东海岸的斑节对虾中呈地方流行性。
初步筛查表明，韦帕可能是未感染GAV和MoV的斑节对虾的来源地。为评估这一点，在韦帕附近捕获的150多只对虾在当地水箱中饲养2周，并作为应激源放血三次以促进更高水平的感染。然后使用实时定量逆转录聚合酶链反应（qRT-PCR）测定淋巴器官组织中GAV和MoV的存在情况。一些对虾在水箱中再饲养75天后进行检测。
实时qRT-PCR在从166只测试对虾中分离的33个RNA样本池中均未检测到GAV。在五个RNA样本池中检测到MoV，其病毒RNA拷贝数极低，接近检测的灵敏度阈值。在用作阴性对照的九个珍珠贝外套膜样本中的一个中也检测到了类似低拷贝数的MoV。
在韦帕近岸水域栖息的幼年斑节对虾中，要么不存在GAV感染，要么像MoV一样，潜在感染率极低。因此，该地区可被推荐为可认证为无GAV和MoV特定病原体的斑节对虾的良好来源地，这对澳大利亚的驯化和选择性育种计划来说是理想的。
