Maurer T, Kimber I
Toxicology Services, Ciba-Geigy Ltd., Basel, Switzerland.
Toxicology. 1991;69(2):209-18. doi: 10.1016/0300-483x(91)90232-p.
The local lymph node assay in the mouse is a novel predictive test for the identification of contact sensitizing chemicals. The purpose of the studies described was to determine whether a similar local lymph node assay could be performed successfully in guinea pigs; currently the species of choice for assessment of sensitizing potential for regulatory purposes. Ten sensitizing chemicals (oxazolone, picryl chloride, 2,4-dinitrofluorobenzene, benzocaine, cinnamic aldehyde, 2,4,-dinitrothiocyanobenzene, p-nitrosodimethylaniline, formaldehyde, p-phenylenediamine and cyanuric chloride) and equal concentrations of sodium lauryl sulphate were examined in a guinea pig local lymph node assay. Animals received three consecutive daily applications of various concentrations of the test chemical on the dorsum of both ears. Control animals were untreated. Five days following the initiation of exposure, draining auricular lymph nodes were excised and weighed. Suspensions of lymph node cells (LNC) were prepared and cultured for 24 or 48 h and proliferation measured by incorporation of [3H]thymidine. Exposure to at least one concentration of all sensitizing chemicals, other than benzocaine, induced proliferation by draining LNC. Responses were higher at 24 h rather than 48 h. Evidence is presented that guinea pig LNC proliferation may be enhanced or maintained by addition to culture of an exogenous source of the T cell growth factor interleukin 2 (IL-2). Draining lymph node weight was increased following exposure to some sensitizing chemicals but, compared with LNC proliferation, provided a less sensitive correlate of lymph node activation. Exposure to sodium lauryl sulphate failed to induce changes in either lymph node weight of LNC proliferation. Data are compared with three-day murine local lymph node assays performed concurrently. The available information indicates that the local lymph node assay may be performed in guinea pigs.
小鼠局部淋巴结试验是一种用于鉴定接触性致敏化学物质的新型预测性试验。所述研究的目的是确定是否能在豚鼠中成功进行类似的局部淋巴结试验；目前豚鼠是出于监管目的评估致敏潜力时的首选物种。在豚鼠局部淋巴结试验中，对10种致敏化学物质（恶唑酮、苦味酰氯、2,4-二硝基氟苯、苯佐卡因、肉桂醛、2,4-二硝基硫氰酸苯酯、对亚硝基二甲基苯胺、甲醛、对苯二胺和三聚氰酰氯）以及相同浓度的十二烷基硫酸钠进行了检测。动物连续三天每天在双耳背部涂抹不同浓度的受试化学物质。对照动物不做处理。暴露开始五天后，切除引流耳淋巴结并称重。制备淋巴结细胞（LNC）悬液并培养24或48小时，通过掺入[3H]胸腺嘧啶核苷来测量增殖情况。除苯佐卡因外，暴露于所有致敏化学物质的至少一种浓度下均能诱导引流LNC增殖。24小时时的反应高于48小时。有证据表明，通过向培养物中添加外源性T细胞生长因子白细胞介素2（IL-2），豚鼠LNC增殖可能会增强或维持。暴露于某些致敏化学物质后，引流淋巴结重量增加，但与LNC增殖相比，其作为淋巴结激活的相关性较低。暴露于十二烷基硫酸钠未能诱导淋巴结重量或LNC增殖发生变化。将数据与同时进行的为期三天的小鼠局部淋巴结试验进行了比较。现有信息表明局部淋巴结试验可在豚鼠中进行。
