Kimber I, Hilton J, Botham P A, Basketter D A, Scholes E W, Miller K, Robbins M C, Harrison P T, Gray T J, Waite S J
ICI Central Toxicology Laboratory, Park, Macclesfield, Cheshire, U.K.
Toxicol Lett. 1991 Feb;55(2):203-13. doi: 10.1016/0378-4274(91)90135-s.
The local lymph node assay is a novel predictive test for the identification of contact allergens. The collaborative study reported here was performed to evaluate the reliability of the method when performed in independent laboratories. Eight chemicals were examined in each of 4 participating laboratories and results compared with predictions of skin-sensitizing activity made from concurrent Magnusson and Kligman guinea-pig maximization tests performed in a single laboratory. The local lymph node assay has as its theoretical basis the fact that contact allergens induce T-lymphocyte proliferative responses. In practice, predictions of contact-sensitizing potential are made following measurement of proliferation in lymph nodes draining the site of exposure to chemical, and derivation of a stimulation index using control values as the comparator. Although in the present study there was some variation between laboratories with respect to the absolute stimulation indices recorded, it was found that with all chemicals each laboratory made the same predictions of sensitizing activity. Six chemicals (2,4-dinitrochlorobenzene, formalin, eugenol, isoeugenol, p-phenylenediamine and potassium dichromate) yielded positive responses, and two (methyl salicylate and benzocaine) were negative, in each laboratory. Furthermore, with 7 of the 8 chemicals tested there was no significant difference between laboratories in terms of the characteristics of the dose-response relationships recorded. With the exception of one chemical (benzocaine), predictions made with the local lymph node assay were in accord with those derived from guinea-pig maximization tests. These inter-laboratory comparisons demonstrate that the local lymph node assay is a robust and reliable method for the identification of at least moderate and strong contact allergens.
局部淋巴结试验是一种用于识别接触性变应原的新型预测性试验。本文报道的协作研究旨在评估该方法在独立实验室进行时的可靠性。4个参与实验室对8种化学物质进行了检测，并将结果与在单个实验室同时进行的Magnusson和Kligman豚鼠最大化试验对皮肤致敏活性的预测结果进行了比较。局部淋巴结试验的理论基础是接触性变应原可诱导T淋巴细胞增殖反应。在实际操作中，通过测量接触化学物质部位引流淋巴结的增殖情况，并以对照值作为比较对象得出刺激指数，从而预测接触致敏潜力。尽管在本研究中，各实验室记录的绝对刺激指数存在一定差异，但发现对于所有化学物质，每个实验室对致敏活性的预测都是相同的。在每个实验室中，6种化学物质（2,4-二硝基氯苯、福尔马林、丁香酚、异丁香酚、对苯二胺和重铬酸钾）产生了阳性反应，2种（水杨酸甲酯和苯佐卡因）为阴性。此外，在检测的8种化学物质中，有7种在各实验室记录的剂量反应关系特征方面没有显著差异。除了一种化学物质（苯佐卡因）外，局部淋巴结试验的预测结果与豚鼠最大化试验得出的结果一致。这些实验室间的比较表明，局部淋巴结试验是一种可靠且稳健的方法，可用于识别至少中度和强接触性变应原。
