Wu Zhiqiang, Yang Fan, Zhao Rong, Zhao Lina, Guo Deyin, Jin Qi
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, No.6 Rongjing East Street, Yi Zhuang, Beijing 100176, China.
J Virol Methods. 2009 Aug;159(2):233-8. doi: 10.1016/j.jviromet.2009.04.002. Epub 2009 Apr 10.
Enterovirus 71 (EV 71) is one of the commonest causative agents of hand, foot, and mouth disease (HFMD), which infects mainly young children. It has been associated with severe neurological complications worldwide, and has caused significant deaths in many provinces of China from March to May 2008. In this study, RNA interference (RNAi) was used as an antiviral agent to inhibit EV 71 replication in rhabdomyosarcoma (RD) cells. Three small interfering RNAs (siRNAs) targeting extremely conserved regions among multiple EV 71 strains in China could effectively block the replication of EV 71 strain Shzh-98. Combination transfection of these three siRNAs could produce a strong inhibitory effect not only in strain Shzh-98, but also in one epidemic strain Fuyang-0805 isolated from a child in the city of Fuyang with a clinical diagnosis of HFMD in 2008. These strategies and results suggest that RNAi has potential therapeutic use for the suppression of EV 71 infection in a broad spectrum of viral strains.
肠道病毒71型（EV 71）是手足口病（HFMD）最常见的病原体之一，主要感染幼儿。在全球范围内，它与严重的神经并发症有关，并且在2008年3月至5月间在中国许多省份导致了大量死亡。在本研究中，RNA干扰（RNAi）被用作抗病毒剂来抑制横纹肌肉瘤（RD）细胞中EV 71的复制。三种靶向中国多个EV 71毒株中高度保守区域的小干扰RNA（siRNAs）能够有效阻断EV 71毒株Shzh - 98的复制。这三种siRNAs的联合转染不仅对Shzh - 98毒株，而且对2008年从阜阳市一名临床诊断为手足口病的儿童中分离出的一株流行毒株Fuyang - 0805都能产生强烈的抑制作用。这些策略和结果表明，RNAi在抑制广泛病毒株的EV 71感染方面具有潜在的治疗用途。
