Sherwood J A, Oster C N, Adoyo-Adoyo M, Beier J C, Gachihi G S, Nyakundi P M, Ballou W R, Brandling-Bennett A D, Schwartz I K, Were J B
US Army Medical Research Unit-Kenya.
Trans R Soc Trop Med Hyg. 1991 May-Jun;85(3):336-40. doi: 10.1016/0035-9203(91)90281-3.
Recombinant sporozoite vaccine or placebo were administered once to 25 volunteers from an area endemic for malaria. Antibody to R32tet32 rose in 9 of 15 receiving vaccine and remained elevated in 6 for 6 months. Mean absorbance increase was 0.43 +/- 0.40 with vaccine, 0.01 +/- 0.23 with placebo, and 0.72 +/- 0.19 in responders. Six non-responders had significantly lower pre-immunization levels (0.07 +/- 0.05) than responders (0.39 +/- 0.25). There was an association between an increase in immunofluorescence (n = 4) and an increase in absorbance (n = 9) among vaccine recipients (n = 15). Vaccine-induced increase in antibody to natural circumsporozoite antigen was indicated by increases in immunofluorescence and by increases in circumsporozoite precipitation score in 2 of the 5 responders with highest antibody increase measured by enzyme-linked immunosorbent assay. Response to subunit sporozoite vaccine paralleled response to prior natural sporozoite exposure and was significant and prolonged in a population with prior natural exposure to malaria.
重组子孢子疫苗或安慰剂被给予来自疟疾流行地区的25名志愿者，每人接种一次。在接种疫苗的15人中，有9人针对R32tet32的抗体上升，其中6人的抗体在6个月内持续升高。接种疫苗者的平均吸光度增加为0.43±0.40，安慰剂组为0.01±0.23，应答者为0.72±0.19。6名无应答者免疫前水平（0.07±0.05）显著低于应答者（0.39±0.25）。在15名疫苗接种者中，免疫荧光增加（n = 4）与吸光度增加（n = 9）之间存在关联。通过免疫荧光增加以及在5名抗体增加最多的应答者中有2人通过酶联免疫吸附测定法测得的环子孢子沉淀评分增加，表明疫苗诱导了针对天然环子孢子抗原的抗体增加。对亚单位子孢子疫苗的反应与先前自然接触子孢子的反应相似，并且在先前有过自然疟疾接触的人群中反应显著且持久。
