Hollingdale M R, Appiah A, Leland P, do Rosario V E, Mazier D, Pied S, Herrington D A, Chulay J D, Ballou W R, Derks T
Biomedical Research Institute, Rockville, MD 20852.
Trans R Soc Trop Med Hyg. 1990 May-Jun;84(3):325-9. doi: 10.1016/0035-9203(90)90303-v.
Sera from human volunteers immunized with either synthetic peptide (NANP)3-TT or recombinant protein R32tet32 Plasmodium falciparum CS vaccines were tested in the inhibition of sporozoite invasion (ISI) assays using human hepatoma (HepG2-A16) cells or primary human hepatocytes. Sera or purified immunoglobulin (Ig) from volunteers who were completely protected against P. falciparum sporozoite challenge had higher ISI activity than sera from non-protected volunteers, or the highest titre endemic serum. However, Ig from protected and non-protected volunteers did not block sporozoite invasion of human hepatocytes, suggesting that P. falciparum sporozoites invade hepatocytes by mechanisms which differ from those concerned with invasion of HepG2-A16 cells.
用合成肽（NANP）3-TT或重组蛋白R32tet32恶性疟原虫环子孢子蛋白（CS）疫苗免疫的人类志愿者的血清，在使用人肝癌（HepG2-A16）细胞或原代人肝细胞的子孢子侵袭抑制（ISI）试验中进行了检测。对恶性疟原虫子孢子攻击完全有保护作用的志愿者的血清或纯化免疫球蛋白（Ig），其ISI活性高于未受保护志愿者的血清或滴度最高的流行区血清。然而，受保护和未受保护志愿者的Ig均未阻断子孢子对人肝细胞的侵袭，这表明恶性疟原虫子孢子侵袭肝细胞的机制不同于其侵袭HepG2-A16细胞的机制。
