Pérez Recalde Mercedes, Noseda Miguel D, Pujol Carlos A, Carlucci María J, Matulewicz María C
Departamento de Química Orgánica-CIHIDECAR (CONICET-UBA), Facultad de Ciencias, Exactas y Naturales, UBA, Pabellón 2, Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
Phytochemistry. 2009 May;70(8):1062-8. doi: 10.1016/j.phytochem.2009.05.001. Epub 2009 Jun 1.
Nemalion helminthoides, collected in the Argentine South Atlantic coast, was extracted with hot water and the crude product fractionated using cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration and seven fractions were separated and analyzed. Structural analysis of the main fractions, those soluble in 3.0 and 4.0 M NaCl (yields 21.0% and 13.8%, respectively) and those insoluble in 4.0 M NaCl (yield 20.0%), indicated that this seaweed biosynthesizes (1-->3)-linked alpha-D-mannans that are sulfated at positions 4 and 6. Three mannan fractions comprising considerable amounts of xylose were also isolated in very low total yield (2.0%). The fractions that were soluble in 3.0 and 4.0 M NaCl showed low antiherpetic activity whereas this activity was considerable for the fraction solubilized in 2.0 M NaCl (yield 0.5%) which contained single stubs of beta-D-xylose. A xylan, soluble in cetrimide solution, containing (1-->3, 1-->4)-linked beta-D-xylose residues, was also isolated in minor amount.
从阿根廷南大西洋海岸采集的蠕枝藻，用热水提取，粗产物用十六烷基三甲基溴化铵分级分离。将络合物质在氯化钠浓度不断增加的溶液中进行分级溶解，分离并分析了七个级分。对主要级分进行结构分析，即那些可溶于3.0和4.0 M氯化钠的级分（产率分别为21.0%和13.8%）以及那些不溶于4.0 M氯化钠的级分（产率20.0%），结果表明这种海藻生物合成了在4位和6位硫酸化的（1→3）连接的α-D-甘露聚糖。还以非常低的总产率（2.0%）分离出了三个含有大量木糖的甘露聚糖级分。可溶于3.0和4.0 M氯化钠的级分显示出低抗疱疹活性，而对于可溶于2.0 M氯化钠（产率0.5%）且含有单个β-D-木糖残基的级分，这种活性相当可观。还少量分离出了一种可溶于十六烷基三甲基溴化铵溶液的木聚糖，其含有（1→3，1→4）连接的β-D-木糖残基。
