Spilker Theodore, Baldwin Adam, Bumford Amy, Dowson Chris G, Mahenthiralingam Eshwar, LiPuma John J
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, 48109-0646, USA.
J Clin Microbiol. 2009 Aug;47(8):2607-10. doi: 10.1128/JCM.00770-09. Epub 2009 Jun 3.
PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.
针对当前洋葱伯克霍尔德菌复合体多位点序列分型方案中各基因座的聚合酶链式反应(PCR)引物被重新设计,目的是:(i)更可靠地从洋葱伯克霍尔德菌复合体菌种中扩增出这些基因座;(ii)从其他伯克霍尔德菌属菌种中扩增出同样的基因座;以及(iii)使得每个基因座能够使用单一引物组进行扩增和DNA测序。