储存的古思里卡片血斑中抗弓形虫免疫球蛋白M的回收情况。
Recovery of anti-Toxoplasma gondii immunoglobulin M in stored guthrie card blood spots.
作者信息
Tan Hooi-Kuan, Petersen Eskild, Møller Lone N, Phillips Pamela, Neto Eurico Camargo, Gilbert Ruth E
机构信息
MRC Centre of Epidemiology for Child Health, Institute of Child Health, London, United Kingdom.
出版信息
J Clin Microbiol. 2009 Aug;47(8):2626-8. doi: 10.1128/JCM.00893-09. Epub 2009 Jun 3.
Abstract
Retrospective testing of neonatal Guthrie card blood spots for specific immunoglobulin M (IgM) can distinguish congenital toxoplasmosis from acquired toxoplasmosis. We determined whether storage temperature reduced IgM detection, using filter paper blood samples "spiked" with anti-Toxoplasma IgM. After 300 days, IgM detection deteriorated with storage at room temperature but not at temperatures of 4 degrees C or lower.
摘要
对新生儿古思里卡片血斑进行特定免疫球蛋白M（IgM）的回顾性检测，可区分先天性弓形虫病和获得性弓形虫病。我们使用添加了抗弓形虫IgM的滤纸血样，确定储存温度是否会降低IgM检测率。300天后，在室温下储存时IgM检测率下降，但在4摄氏度或更低温度下储存时则不然。
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