Sardarian Khosro, Maghsood Amir Hossein, Farimani Marzieh, Hajiloii Mehrdad, Saidijam Massoud, Farahpour Manizheh, Mahaki Hanieh, Zamani Alireza
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Hum Antibodies. 2019;27(1):31-35. doi: 10.3233/HAB-180346.
Congenital toxoplasmosis is an important disease that occurs when pregnant women become infected with Toxoplasma gondii during pregnancy. The aim of this study was to investigate the presence of T. gondii B1 gene in placental tissues of IgM seronegative women.
In this research, chronic toxoplasmosis was identified through examination of blood samples in a group of pregnant women by anti-Toxoplasma IgG and IgM ELISA and nested-PCR techniques. IgG avidity test was used to estimate the onset of infection in some pregnant women with chronic infection. After delivery, some umbilical cord and neonatal blood were tested by anti-Toxoplasma IgM ELISA, and also the B1 gene of T. gondii was investigated in their placental tissue by nested-PCR. Some factors such as blood sampling time and some clinical symptoms experienced during pregnancy were recorded.
One hundred and sixty seven out of 653 (25.6%) pregnant women were positive for anti-Toxoplasma IgG. Of them, 165 (98.8%) were negative for anti-T. gondii IgM. Six out of 10 (60%) placental tissues from IgG seropositive, IgM seronegative women were positive for T. gondii B1 gene, while anti-Toxoplasma IgM was negative in the umbilical cord and neonatal blood samples. The results of IgG avidity test showed low avidity in one and high avidity in two women's samples. The B1 gene was not found in the blood samples of any of the six mothers. The most symptoms experienced during pregnancy were headache and nausea.
The detection of B1 gene in placental tissues of the healthy newborn infants reiterates that presence of T. gondii in the placenta does not always result in congenital toxoplasmosis.
先天性弓形虫病是一种重要疾病，发生于孕妇在孕期感染弓形虫时。本研究旨在调查IgM血清学阴性女性胎盘组织中弓形虫B1基因的存在情况。
在本研究中，通过抗弓形虫IgG和IgM酶联免疫吸附测定（ELISA）以及巢式聚合酶链反应（PCR）技术对一组孕妇的血样进行检测，以识别慢性弓形虫病。IgG亲和力试验用于估计一些慢性感染孕妇的感染发病时间。分娩后，通过抗弓形虫IgM ELISA对一些脐带血和新生儿血样进行检测，同时通过巢式PCR在其胎盘组织中研究弓形虫的B1基因。记录一些因素，如采血时间以及孕期经历的一些临床症状。
653名孕妇中有167名（25.6%）抗弓形虫IgG呈阳性。其中，165名（98.8%）抗弓形虫IgM呈阴性。10名IgG血清学阳性、IgM血清学阴性女性的胎盘组织中有6名（60%）弓形虫B1基因呈阳性，而脐带血和新生儿血样中的抗弓形虫IgM呈阴性。IgG亲和力试验结果显示，一名女性样本的亲和力低，两名女性样本的亲和力高。在这六名母亲的任何血样中均未发现B1基因。孕期最常出现的症状是头痛和恶心。
在健康新生儿的胎盘组织中检测到B1基因，再次表明胎盘中存在弓形虫并不一定会导致先天性弓形虫病。
