Koblov N L, Suskova V S, Perepanova T S, Onishchenko N A, Danilkov A P, Orzhekhovskaia I G
Urol Nefrol (Mosk). 1991 Jul-Aug(4):25-30.
Hemoperfusion through cryopreserved fragments of porcine xenospleen tissue was used in combined treatment of 42 patients with pyoseptic urologic diseases: urosepsis, purulent intoxication due to acute pyelonephritis, pyoinflammatory scrotal and prostatic lesions (in 15, 19, 8 patients, respectively). Indications and contraindications are specified. Altogether 57 sessions were performed. Seventeen patients underwent the perfusion pre- and postoperatively, twenty-five in combination with chemotherapy. After the removal porcine spleen was dissected, treated with cryoprotective mixture (-196 degrees C) and placed into liquid nitrogen. One-year viability of the tissue retained in 82-93% of the samples. Duration of the hemoperfusion was 40-80 min, the rate--30-40 ml/min. Two-four columns with cryopreserved tissue were utilized which were alternately connected with the circuit. Heparinization reached 12,000-15,000 units. All the patients survived. Positive clinical and laboratory findings were obtained for 38 patients (90%). Urine and blood samples appeared sterile on day 7-12. The improvement of the severe condition of the patients is attributed to detoxication, immunostimulation and immunocorrective effects.
通过猪异种脾组织冷冻保存片段进行血液灌流用于42例脓性泌尿系统疾病患者的联合治疗：分别为尿脓毒症、急性肾盂肾炎所致的脓性中毒、阴囊和前列腺脓性炎症病变（分别为15例、19例、8例患者）。明确了适应证和禁忌证。共进行了57次治疗。17例患者在术前和术后进行了灌流，25例与化疗联合进行。猪脾取出后进行解剖，用冷冻保护剂处理（-196℃）并置于液氮中。82% - 93%的样本中组织的一年存活率得以保留。血液灌流持续时间为40 - 80分钟，速率为30 - 40毫升/分钟。使用了两到四根装有冷冻保存组织的柱体，它们交替连接到循环回路中。肝素化达到12000 - 15000单位。所有患者均存活。38例患者（90%）获得了阳性的临床和实验室结果。在第7 - 12天，尿液和血液样本无菌。患者严重病情的改善归因于解毒、免疫刺激和免疫纠正作用。
