Boosman R, Mutsaers C W, Klarenbeek A
Department of General and Large Animal Surgery, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Vet Q. 1991 Jul;13(3):155-62. doi: 10.1080/01652176.1991.9694301.
To study the possible role of endotoxin in the pathogenesis of bovine laminitis, local and systemic injections of endotoxin (E. coli 0111 B4) with different doses were given to three groups of four cows each. Clinical and haematologic parameters indicated an acute-phase response, including positive plasma ethanol gelation (soluble fibrin), the occurrence of fibrin degradation products and decreased thrombocyte counts. Local Shwartzman reactions were not evoked. Clinical examination of the claws and the gait of the animals revealed no signs of laminitis. However, on histopathological examination of the claw corium signs of laminitis such as vacuolisation of the Stratum basale, lymphocyte and leucocyte infiltration and thrombosis were found. These results indicate that endotoxin indeed may be involved in the pathogenesis of laminitis. For the development of a clinical acute laminitis model in cattle either another dosage, other toxins or factors in addition to the endotoxin used in this experiment are needed.
为研究内毒素在牛蹄叶炎发病机制中的可能作用，将不同剂量的内毒素（大肠杆菌0111 B4）分别局部和全身注射到三组牛中，每组四头。临床和血液学参数显示存在急性期反应，包括血浆乙醇凝胶化阳性（可溶性纤维蛋白）、纤维蛋白降解产物的出现以及血小板计数降低。未诱发局部施瓦茨曼反应。对动物蹄爪和步态的临床检查未发现蹄叶炎迹象。然而，在对蹄真皮进行组织病理学检查时，发现了蹄叶炎的迹象，如基底层空泡化、淋巴细胞和白细胞浸润以及血栓形成。这些结果表明内毒素确实可能参与了蹄叶炎的发病机制。要建立牛临床急性蹄叶炎模型，可能需要本实验中使用的内毒素以外的其他剂量、其他毒素或因素。
