Wilkinson R, Allanson M, Kolega V, Lawrence D, Neville S
Central Veterinary Laboratories (VETLAB), Department of Agriculture, Adelaide, S.A. Australia.
Vet Immunol Immunopathol. 1991 Aug;29(1-2):189-95. doi: 10.1016/0165-2427(91)90064-j.
The production of koala immunoglobulins (Ig) was elicited by the immunisation of a koala (Phascolarctos cinereus) with bovine serum albumin. This Ig was then purified using the highly specific techniques of affinity chromatography. The purified protein was compared with a "potential" koala Ig protein which was subsequently purified by Protein G chromatography and both proteins were further characterised by agarose/SDS-PAGE and immunoelectrophoresis. Results indicate that koalas do produce Ig in response to antigen challenge, koala Ig has a higher net negative charge than that seen in most other mammals and possibly two subclasses of IgG and IgM are present in normal koala serum.
通过用牛血清白蛋白免疫考拉（树袋熊）来引发考拉免疫球蛋白（Ig）的产生。然后使用亲和色谱这种高度特异性的技术对这种Ig进行纯化。将纯化后的蛋白质与随后通过蛋白G色谱法纯化的“潜在”考拉Ig蛋白进行比较，并且通过琼脂糖/SDS-聚丙烯酰胺凝胶电泳和免疫电泳对这两种蛋白质进行进一步表征。结果表明，考拉确实会在抗原刺激下产生Ig，考拉Ig的净负电荷比大多数其他哺乳动物中的Ig更高，并且正常考拉血清中可能存在IgG和IgM的两个亚类。
