Woolard Kevin, Fine Howard A
Neuro-Oncology Branch, National Cancer Institute, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Cell Stem Cell. 2009 Jun 5;4(6):466-7. doi: 10.1016/j.stem.2009.05.013.
Glioma-initiating cells/glioma stem cells (GIC/GSCs) are grown in vitro using a cumbersome and inefficient spheroid assay. In this issue of Cell Stem Cell, Pollard and coworkers present a protocol for the efficient derivation of GIC/GSC lines that may greatly improve the isolation and the potential clinical application of these cells.
胶质瘤起始细胞/胶质瘤干细胞(GIC/GSCs)在体外培养时采用的是一种繁琐且低效的球体检测法。在本期《细胞干细胞》杂志中,波拉德及其同事提出了一种高效获取GIC/GSC系的方案,这可能会极大地改善这些细胞的分离及其潜在的临床应用。