Dai Niann-Tzyy, Yeh Ming-Kung, Chiang Chiao-Hsi, Chen Ke-Chi, Liu Tsung-Hsun, Feng An-Chieh, Chao Li-Lien, Shih Chien-Ming, Sytwu Huey-Kang, Chen Shao-Liang, Chen Tim-Mo, Adams E F
Division of Plastic and Reconstructive Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taiwan, ROC.
Biochem Biophys Res Commun. 2009 Aug 14;386(1):21-5. doi: 10.1016/j.bbrc.2009.05.123. Epub 2009 Jun 2.
The development and characterization of an enhanced composite skin substitute based on collagen and poly(epsilon-caprolactone) are reported. Considering the features of excellent biocompatibility, easy-manipulated property and exempt from cross-linking related toxicity observed in the 1:20 biocomposites, skin substitutes were developed by seeding human single-donor keratinocytes and fibroblasts alone on both sides of the 1:20 biocomposite to allow for separation of two cell types and preserving cell signals transmission via micro-pores with a porosity of 28.8 +/- 16.1 microm. The bi-layered skin substitute exhibited both differentiated epidermis and fibrous dermis in vitro. Less Keratinocyte Growth Factor production was measured in the co-cultured skin model compared to fibroblast alone condition indicating a favorable microenvironment for epidermal homeostasis. Moreover, fast wound closure, epidermal differentiation, and abundant dermal collagen deposition were observed in composite skin in vivo. In summary, the beneficial characteristics of the new skin substitutes exploited the potential for pharmaceutical screening and clinical application.
报道了一种基于胶原蛋白和聚（ε-己内酯）的增强型复合皮肤替代物的开发与特性。考虑到在1:20生物复合材料中观察到的优异生物相容性、易于操作的特性以及无交联相关毒性等特点，通过在1:20生物复合材料的两侧单独接种人类单供体角质形成细胞和成纤维细胞来开发皮肤替代物，以实现两种细胞类型的分离，并通过孔隙率为28.8±16.1微米的微孔保持细胞信号传递。这种双层皮肤替代物在体外表现出分化的表皮和纤维状真皮。与单独培养成纤维细胞的条件相比，在共培养皮肤模型中检测到的角质形成细胞生长因子产量较低，这表明存在有利于表皮稳态的微环境。此外，在体内复合皮肤中观察到伤口快速闭合、表皮分化和丰富的真皮胶原沉积。总之，新型皮肤替代物的有益特性为药物筛选和临床应用开发了潜力。
