Jeon Yeji, Kim Jeong Myeong, Park Jin Ho, Lee Se Hee, Seong Chi-Nam, Lee Sang-Suk, Jeon Che Ok
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2491-5. doi: 10.1099/ijs.0.005710-0. Epub 2009 Jul 21.
A Gram-stain-negative, strictly aerobic bacterium, designated strain N7(T), was isolated from a rice paddy in South Korea. Cells of strain N7(T) were non-motile, non-spore-forming rods. Growth was observed at 15-35 degrees C (optimum of 25-30 degrees C) and between pH 6.0 and 8.0 (optimum of pH 6.5-7.5). The predominant isoprenoid quinone was menaquinone-7. The major cellular fatty acids of strain N7(T) were summed feature 3 (comprising C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), iso-C(15 : 0), anteiso-C(15 : 0), C(15 : 0) and iso-C(16 : 0). The G+C content of the genomic DNA was 37.7 mol%. Comparative 16S rRNA gene sequence analyses showed that strain N7(T) formed a distinct phyletic line within the genus Pedobacter. Phylogenetic distances from strains of other Pedobacter species with validly published names were greater than 5.0 % (i.e. <95.0 % 16S rRNA gene sequence similarities). On the basis of phenotypic and molecular data, it is clear that strain N7(T) represents a novel species within the genus Pedobacter, for which the name Pedobacter oryzae sp. nov. is proposed. The type strain is N7(T) (=KACC 12821(T) =DSM 19973(T)).
从韩国的一块稻田中分离出了一株革兰氏染色阴性、严格需氧的细菌，命名为菌株N7(T)。菌株N7(T)的细胞为无运动性、不形成芽孢的杆状。在15 - 35℃（最适温度为25 - 30℃）和pH 6.0至8.0（最适pH为6.5 - 7.5）之间可观察到生长。主要的类异戊二烯醌为甲基萘醌-7。菌株N7(T)的主要细胞脂肪酸为总和特征3（包含C(16 : 1)ω7c和/或异-C(15 : 0) 2-OH）、异-C(15 : 0)、anteiso-C(15 : 0)、C(15 : 0)和异-C(16 : 0)。基因组DNA的G+C含量为37.7 mol%。比较16S rRNA基因序列分析表明，菌株N7(T)在Pedobacter属内形成了一个独特的进化分支。与其他已有效发表名称的Pedobacter属菌株的系统发育距离大于5.0%（即16S rRNA基因序列相似性<95.0%）。基于表型和分子数据，很明显菌株N7(T)代表Pedobacter属内的一个新物种，为此提出名为Pedobacter oryzae sp. nov.的名称。模式菌株为N7(T)（=KACC 12821(T) =DSM 19973(T)）。
