Duangmal Kannika, Thamchaipenet Arinthip, Matsumoto Atsuko, Takahashi Yoko
Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1487-91. doi: 10.1099/ijs.0.007724-0.
A novel Gram-positive-staining actinomycete designated strain GMKU095(T) was isolated from surface-sterilized roots of Acacia auriculiformis A. Cunn. ex Benth. (earpod wattle). The organism produced branching mycelium. The spores were non-motile and had a spiny surface. Growth of strain GMKU095(T) occurred at 18-42 degrees C, pH 5.0-8.0 and at NaCl concentrations up to 5 %. Whole-cell hydrolysates contained arabinose and galactose as major characteristic sugars. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The menaquinone was MK-8(H(4)); mycolic acids were not detected. The G+C content of the DNA was 71.6 mol%. iso-C(16 : 0) was detected as the major cellular fatty acid. Comparative studies of 16S rRNA gene sequences indicated that the strain was phylogenetically related to members of the genus Pseudonocardia. The most closely related type strain is Pseudonocardia spinosispora IMSNU 50581(T), which is 96.2 % similar in 16S rRNA gene sequence. On the basis of the genotypic and phenotypic properties presented, a novel species of the genus Pseudonocardia is proposed, Pseudonocardia acaciae sp. nov. The type strain is strain GMKU095(T) (=NRRL B-24609(T) =BCC 28481(T) =TISTR 1862(T) =NBRC 104274(T)).
从表面消毒的耳荚相思树（Acacia auriculiformis A. Cunn. ex Benth.）根部分离出一株新的革兰氏阳性放线菌，命名为GMKU095(T)。该菌产生分支菌丝体。孢子无运动性，表面有刺。GMKU095(T)菌株在18 - 42℃、pH 5.0 - 8.0以及NaCl浓度高达5%的条件下生长。全细胞水解产物中主要特征性糖为阿拉伯糖和半乳糖。肽聚糖的诊断性二氨基酸为内消旋二氨基庚二酸。胞壁质的聚糖部分含有乙酰残基。甲基萘醌为MK - 8(H(4))；未检测到分枝菌酸。DNA的G + C含量为71.6 mol%。检测到iso - C(16 : 0)为主要细胞脂肪酸。16S rRNA基因序列的比较研究表明，该菌株在系统发育上与假诺卡氏菌属成员相关。最密切相关的模式菌株是棘孢假诺卡氏菌（Pseudonocardia spinosispora）IMSNU 50581(T)，其16S rRNA基因序列相似度为96.2%。基于所呈现的基因型和表型特征，提出假诺卡氏菌属的一个新物种——相思假诺卡氏菌（Pseudonocardia acaciae）。模式菌株为GMKU095(T)（=NRRL B - 24609(T) =BCC 28481(T) =TISTR 1862(T) =NBRC 104274(T)）。
