Suriyachadkun Chanwit, Chunhametha Suwanee, Thawai Chitti, Tamura Tomohiko, Potacharoen Wanchern, Kirtikara Kanyawim, Sanglier Jean-Jacques
BIOTEC Culture Collection (BCC), BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani 12120, Thailand.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):992-7. doi: 10.1099/ijs.0.003228-0.
A Gram-positive-staining, filamentous bacterial strain that developed cylindrical sporangia containing four oval- to rod-shaped spores at the ends of short sporangiophores on branched aerial mycelium was isolated from tropical rainforest soil near a hot spring. The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; the whole-cell hydrolysate contained rhamnose, madurose, glucose, galactose and 3-O-methylmannose as whole-cell sugars. The predominant menaquinone was MK-9(H(4)). Mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were iso-C(16 : 0) and 10-methylated C(17 : 0). The G+C content of the DNA was 71.1 mol%. The phenotypic and chemotaxonomic analyses showed that the isolate had characteristics typical of members of the genus Planotetraspora. Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belongs to the genus Planotetraspora, but as a putative novel species. Following phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to be a representative of a novel species, to be named Planotetraspora thailandica sp. nov. The type strain is BCC 21825(T) (=NBRC 104271(T)). An emended description of the genus Planotetraspora is also presented.
从温泉附近的热带雨林土壤中分离出一株革兰氏阳性丝状细菌菌株，该菌株在分支气生菌丝体的短孢子囊梗末端形成含有四个椭圆形至杆状孢子的圆柱形孢子囊。细胞壁肽聚糖含有内消旋二氨基庚二酸、谷氨酸和丙氨酸作为细胞壁氨基酸；全细胞水解产物含有鼠李糖、马杜拉糖、葡萄糖、半乳糖和3-O-甲基甘露糖作为全细胞糖类。主要的甲基萘醌是MK-9(H(4))。未检测到分枝菌酸。诊断性磷脂是磷脂酰乙醇胺。主要的细胞脂肪酸是异-C(16 : 0)和10-甲基化C(17 : 0)。DNA的G+C含量为71.1 mol%。表型和化学分类分析表明，该分离株具有扁平四孢菌属成员的典型特征。此外，16S rRNA基因序列分析也表明该菌株属于扁平四孢菌属，但可能是一个新物种。经过表型、化学分类和基因型研究，该分离株被提议作为一个新物种的代表，命名为泰国扁平四孢菌（Planotetraspora thailandica sp. nov.）。模式菌株是BCC 21825(T)（=NBRC 104271(T)）。还给出了扁平四孢菌属的修订描述。
