Li Jie, Zhao Guo-Zhen, Qin Sheng, Zhu Wen-Yong, Xu Li-Hua, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1492-6. doi: 10.1099/ijs.0.007534-0.
An endophytic actinomycete, strain YIM 65188(T), was isolated from surface-sterilized tissue of Sedum sp. collected from Yunnan province, south-west China, and characterized by using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain YIM 65188(T) is a member of the genus Streptomyces and exhibited 97.5 and 96.3 % gene sequence similarities to Streptomyces specialis GW41-1564(T) and Streptomyces hainanensis YIM 47672(T), respectively, whereas low sequence similarity values (<95.2 %) distinguished strain YIM 65188(T) from all other Streptomyces species with validly published names. Strain YIM 65188(T) had a unique menaquinone composition, with the predominant quinones being MK-11(H(6)) and MK-10(H(6)), with moderate amounts of MK-10(H(8)), MK-11(H(8)), MK-9(H(8)), MK-11(H(4)), MK-9(H(6)) and MK-10(H(4)). Similarly, the closest phylogenetic relative, S. specialis GW41-1564(T), also had an unusual quinone composition, with the predominant menaquinones MK-10(H(4)) and MK-10(H(6)) and minor amounts of MK-9(H(4)) and MK-9(H(6)). The DNA-DNA hybridization value between strain YIM 65188(T) and S. specialis GW41-1564(T) was 42.7 %. On the basis of phenotypic and phylogenetic characteristics, strain YIM 65188(T) was identified as a novel species of the genus Streptomyces for which the name Streptomyces sedi sp. nov. is proposed, with YIM 65188(T) (=CCTCC AA 208020(T) =DSM 41942(T)) as the type strain.
从中国西南部云南省采集的景天属植物经表面消毒的组织中分离出一株内生放线菌，菌株YIM 65188(T)，并采用多相分类法对其进行了表征。16S rRNA基因序列分析表明，菌株YIM 65188(T)是链霉菌属的成员，与特殊链霉菌GW41-1564(T)和海南链霉菌YIM 47672(T)的基因序列相似性分别为97.5%和96.3%，而低序列相似性值(<95.2%)将菌株YIM 65188(T)与所有其他已有效发表名称的链霉菌物种区分开来。菌株YIM 65188(T)具有独特的甲基萘醌组成，主要醌类为MK-11(H(6))和MK-10(H(6))，还有适量的MK-10(H(8))、MK-11(H(8))、MK-9(H(8))、MK-11(H(4))、MK-9(H(6))和MK-10(H(4))。同样，亲缘关系最近的菌株，特殊链霉菌GW41-1564(T)，也有不寻常的醌组成，主要甲基萘醌为MK-10(H(4))和MK-10(H(6))，少量为MK-9(H(4))和MK-9(H(6))。菌株YIM 65188(T)与特殊链霉菌GW41-1564(T)之间的DNA-DNA杂交值为42.7%。基于表型和系统发育特征，菌株YIM 65188(T)被鉴定为链霉菌属的一个新物种，提议将其命名为景天链霉菌（Streptomyces sedi sp. nov.），模式菌株为YIM 65188(T) (=CCTCC AA 208020(T) =DSM 41942(T))。
