Chen Hua-Hong, Zhao Guo-Zhen, Park Dong-Jin, Zhang Yu-Qin, Xu Li-Hua, Lee Jae-Chan, Kim Chang-Jin, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1070-5. doi: 10.1099/ijs.0.006296-0.
A Gram-positive bacterial strain, designated YIM 56238(T), was isolated from plant roots (Aquilaria sinensis), and characterized by using a polyphasic approach. Strain YIM 56238(T) grew optimally at pH 7.0-8.0 and at 28 degrees C. Analysis of the 16S rRNA gene sequence of strain YIM 56238(T) indicated that it belongs to the genus Micrococcus. Chemotaxonomic data strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine and glycine; the predominant menaquinones were MK-8(H(2)) (63.6 %) and MK-7(H(2)) (21.1 %); the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid; and the major cellular fatty acids were iso-C(15 : 0) (30.95 %) and anteiso-C(15 : 0) (53.75 %). The G+C content of the genomic DNA was 72.9 mol%. A number of physiological features were found that clearly distinguished strain YIM 56238(T) from recognized species of the genus Micrococcus. DNA-DNA hybridization studies suggested that the novel strain represents a separate genomic species. On the basis of the data, therefore, strain YIM 56238(T) represents a novel species of the genus Micrococcus, for which the name Micrococcus endophyticus sp. nov. is proposed. The type strain is YIM 56238(T) (=DSM 17945(T)=KCTC 19156(T)).
从植物根部（白木香）分离出一株革兰氏阳性细菌菌株，命名为YIM 56238(T)，并采用多相分类法对其进行了特征描述。菌株YIM 56238(T)在pH 7.0 - 8.0和28℃条件下生长最佳。对菌株YIM 56238(T)的16S rRNA基因序列分析表明，它属于微球菌属。化学分类学数据有力地支持了该菌株在微球菌属内的分类：细胞壁肽聚糖含有赖氨酸、谷氨酸、丙氨酸和甘氨酸；主要的甲基萘醌是MK - 8(H₂)（63.6%）和MK - 7(H₂)（21.1%）；磷脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和一种未知的茚三酮阴性磷脂；主要的细胞脂肪酸是异-C₁₅:₀（30.95%）和anteiso-C₁₅:₀（53.75%）。基因组DNA的G+C含量为72.9 mol%。发现了许多生理特征，这些特征明显将菌株YIM 56238(T)与微球菌属已确认的物种区分开来。DNA - DNA杂交研究表明，该新菌株代表一个独立的基因组物种。因此，根据这些数据，菌株YIM 56238(T)代表微球菌属的一个新物种，为此提出新名称内生微球菌（Micrococcus endophyticus sp. nov.）。模式菌株为YIM 56238(T)（=DSM 17945(T)=KCTC 19156(T)）。
