Bloedt Kathrin, Riecker Melanie, Poppert Sven, Wellinghausen Nele
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany.
Department of Molecular Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
J Med Microbiol. 2009 Jul;58(Pt 7):874-877. doi: 10.1099/jmm.0.009811-0. Epub 2009 Jun 5.
Two new Clostridium difficile-selective agars, from Oxoid (according to Brazier) and from BD, were compared with cycloserine-cefoxitin-fructose agar (Oxoid) for their sensitivity of recovery of toxigenic C. difficile from stool samples. For the culture-positive samples, the sensitivities were 84.0, 42.6 and 90.4 %, respectively. In addition, a C. difficile-specific fluorescence in situ hybridization assay was developed, facilitating rapid and reliable identification of cultured isolates.
将来自Oxoid（根据布雷齐尔的方法）和BD公司的两种新型艰难梭菌选择性琼脂与环丝氨酸-头孢西丁-果糖琼脂（Oxoid）进行比较，以评估它们从粪便样本中回收产毒艰难梭菌的敏感性。对于培养阳性样本，敏感性分别为84.0%、42.6%和90.4%。此外，还开发了一种艰难梭菌特异性荧光原位杂交检测方法，有助于快速、可靠地鉴定培养的分离株。
