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Use of a selective enrichment broth to recover Clostridium difficile from stool swabs stored under different conditions.使用选择性富集肉汤从在不同条件下储存的粪便拭子中回收艰难梭菌。
J Clin Microbiol. 2005 Oct;43(10):5341-3. doi: 10.1128/JCM.43.10.5341-5343.2005.
2
Comparison of selective media for optimal recovery of Clostridium difficile from diarrhoeal stools.用于从腹泻粪便中最佳回收艰难梭菌的选择性培养基的比较。
Indian J Med Res. 1992 Jul;95:181-3.
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Improved recovery of Clostridium difficile spores with the incorporation of synthetic taurocholate in cycloserine-cefoxitin-fructose agar (CCFA).在环丝氨酸头孢西丁果糖琼脂（CCFA）中加入合成牛磺胆酸钠可提高艰难梭菌孢子的回收率。
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Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media.使用两种选择性培养基对水和粪便中艰难梭菌进行分离和定量。
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Comparison of cycloserine-cefoxitin-fructose agar (CCFA) and taurocholate-CCFA for recovery of Clostridium difficile during surveillance of hospitalized patients.在住院患者监测期间，比较环丝氨酸-头孢西丁-果糖琼脂（CCFA）和牛磺胆酸盐-CCFA用于艰难梭菌分离培养的效果。
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Role of culture and toxin detection in laboratory testing for diagnosis of Clostridium difficile-associated diarrhea.培养和毒素检测在艰难梭菌相关性腹泻诊断实验室检测中的作用
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本文引用的文献

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Clostridium difficile-associated diarrhea in a region of Quebec from 1991 to 2003: a changing pattern of disease severity.1991年至2003年魁北克某地区艰难梭菌相关性腹泻：疾病严重程度的变化模式
CMAJ. 2004 Aug 31;171(5):466-72. doi: 10.1503/cmaj.1041104.
2
C. difficile hits Sherbrooke, Que., hospital: 100 deaths.艰难梭菌侵袭魁北克省舍布鲁克市的医院：100人死亡。
CMAJ. 2004 Aug 31;171(5):436. doi: 10.1503/cmaj.1041250.
3
A European survey of diagnostic methods and testing protocols for Clostridium difficile.一项关于艰难梭菌诊断方法和检测方案的欧洲调查。
Clin Microbiol Infect. 2003 Oct;9(10):989-96. doi: 10.1046/j.1469-0691.2003.00683.x.
4
Recent emergence of an epidemic clindamycin-resistant clone of Clostridium difficile among Polish patients with C. difficile-associated diarrhea.近期，在波兰患有艰难梭菌相关性腹泻的患者中出现了一种对克林霉素耐药的流行艰难梭菌克隆。
J Clin Microbiol. 2003 Sep;41(9):4184-7. doi: 10.1128/JCM.41.9.4184-4187.2003.
5
The effects of storage conditions on viability of Clostridium difficile vegetative cells and spores and toxin activity in human faeces.储存条件对艰难梭菌营养细胞和芽孢活力以及人粪便中毒素活性的影响。
J Clin Pathol. 2003 Feb;56(2):126-8. doi: 10.1136/jcp.56.2.126.
6
Nosocomial outbreak of Clostridium difficile-associated diarrhoea due to a clindamycin-resistant enterotoxin A-negative strain.由一株耐克林霉素的A 型阴性肠毒素艰难梭菌引起的医院内艰难梭菌相关性腹泻暴发。
Eur J Clin Microbiol Infect Dis. 2001 Aug;20(8):528-34. doi: 10.1007/s100960100550.
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Epidemiology, risk factors and treatments for antibiotic-associated diarrhea.抗生素相关性腹泻的流行病学、危险因素及治疗方法。
Dig Dis. 1998 Sep-Oct;16(5):292-307. doi: 10.1159/000016879.
8
Comparison of cycloserine-cefoxitin-fructose agar (CCFA) and taurocholate-CCFA for recovery of Clostridium difficile during surveillance of hospitalized patients.在住院患者监测期间，比较环丝氨酸-头孢西丁-果糖琼脂（CCFA）和牛磺胆酸盐-CCFA用于艰难梭菌分离培养的效果。
Diagn Microbiol Infect Dis. 1997 Sep;29(1):1-4. doi: 10.1016/s0732-8893(97)00113-2.
9
Use of cycloserine-cefoxitin-fructose agar and L-proline-aminopeptidase (PRO Discs) in the rapid identification of Clostridium difficile.环丝氨酸-头孢西丁-果糖琼脂和L-脯氨酸氨基肽酶（PRO试纸）在艰难梭菌快速鉴定中的应用
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10
Prevalence and pathogenicity of Clostridium difficile in hospitalized patients. A French multicenter study.住院患者艰难梭菌的患病率及致病性。一项法国多中心研究。
Arch Intern Med. 1996 Jul 8;156(13):1449-54.

使用选择性富集肉汤从在不同条件下储存的粪便拭子中回收艰难梭菌。

Use of a selective enrichment broth to recover Clostridium difficile from stool swabs stored under different conditions.

作者信息

Arroyo Luis G, Rousseau Joyce, Willey Barbara M, Low Don E, Staempfli Henry, McGeer Allison, Weese J Scott

机构信息

Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

出版信息

J Clin Microbiol. 2005 Oct;43(10):5341-3. doi: 10.1128/JCM.43.10.5341-5343.2005.

DOI:10.1128/JCM.43.10.5341-5343.2005
PMID:16208013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1248507/
Abstract

The recovery of Clostridium difficile from the stools of patients with C. difficile-associated diarrhea was evaluated by use of an enrichment broth (cycloserine-cefoxitin fructose broth supplemented with 0.1% sodium taurocholate [TCCFB]) and was compared to that from selective agar (cycloserine-cefoxitin fructose agar [CCFA]) and alcohol shock followed by inoculation onto blood agar (AS-BA). TCCFB was superior to CCFA and AS-BA, and neither the storage time nor the storage temperature affected the recovery rate.

摘要

通过使用一种增菌肉汤（添加0.1%牛磺胆酸钠的环丝氨酸-头孢西丁-果糖肉汤[TCCFB]）评估艰难梭菌相关性腹泻患者粪便中艰难梭菌的回收率，并与选择性琼脂（环丝氨酸-头孢西丁-果糖琼脂[CCFA]）以及酒精休克后接种到血琼脂（AS-BA）上的回收率进行比较。TCCFB优于CCFA和AS-BA，并且储存时间和储存温度均不影响回收率。